Xue Mei, Zhao Yan, Hu Shunlei, Shi Xingming, Cui Hongyu, Wang Yunfeng
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150001, People's Republic of China.
National Engineering Research Center of Veterinary Biologics, Harbin, People's Republic of China.
Arch Virol. 2017 May;162(5):1187-1199. doi: 10.1007/s00705-016-3180-5. Epub 2017 Jan 17.
Infection with reticuloendotheliosis virus (REV), a gammaretrovirus in the family Retroviridae, can result in immunosuppression and subsequent increased susceptibility to secondary infections. In the present study, we identified differentially expressed proteins in the spleens of chickens infected with the REV-A HLJ07I strain, using two-dimensional gel electrophoresis on samples from time points coinciding with different phases of the REV life cycle. Differentially expressed proteins were identified using one-dimensional liquid chromatography electrospray ionization tandem mass spectrometry (1D LC ESI MS/MS). Comparative analysis of multiple gels revealed that the majority of changes occurred at early stages of infection. In total, 60 protein spots representing 28 host proteins were detected as either quantitatively (false discovery rate [FDR] ≤0.05 and fold change ≥2) or qualitatively differentially expressed at least once during different sampling points. The differentially expressed proteins identified in this study included antioxidants, molecular chaperones, cellular metabolism, formation of the cytoskeleton, signal transduction, cell proliferation and cellar aging. The present findings provide a basis for further studies to elucidate the role of these proteins in REV-host interactions. This could lead to a better understanding of REV infection mechanisms that cause immune suppression.
感染网状内皮组织增殖病病毒（REV，一种逆转录病毒科的γ逆转录病毒）可导致免疫抑制，并随后增加对继发感染的易感性。在本研究中，我们使用二维凝胶电泳技术，对来自与REV生命周期不同阶段相吻合的时间点的样本进行分析，从而鉴定出感染REV-A HLJ07I毒株的鸡脾脏中差异表达的蛋白质。利用一维液相色谱电喷雾电离串联质谱（1D LC ESI MS/MS）对差异表达的蛋白质进行鉴定。对多个凝胶的比较分析表明，大多数变化发生在感染的早期阶段。总共检测到60个代表28种宿主蛋白的蛋白点，这些蛋白点在不同采样点至少有一次在定量（错误发现率[FDR]≤0.05且变化倍数≥2）或定性方面存在差异表达。本研究中鉴定出的差异表达蛋白包括抗氧化剂、分子伴侣、细胞代谢、细胞骨架形成、信号转导、细胞增殖和细胞衰老。本研究结果为进一步研究阐明这些蛋白在REV-宿主相互作用中的作用提供了基础。这可能有助于更好地理解导致免疫抑制的REV感染机制。
