Esteban-Manzanares Gustavo, González-Bermúdez Blanca, Cruces Julia, De la Fuente Mónica, Li Qingxuan, Guinea Gustavo V, Pérez-Rigueiro José, Elices Manuel, Plaza Gustavo R
Center for Biomedical Technology. Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Spain.
Departamento de Ciencia de Materiales, ETSI de Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain.
Ann Biomed Eng. 2017 May;45(5):1375-1385. doi: 10.1007/s10439-017-1795-7. Epub 2017 Jan 17.
Mechanical deformability of cells is an important property for their function and development, as well as a useful marker of cell state. The classical technique of micropipette aspiration allows single-cell studies and we provide here a method to measure the two basic mechanical parameters, elastic modulus and Poisson's ratio. The proposed method, developed from finite-element analysis of micropipette aspiration experiments, may be implemented in future technologies for the automated measurement of mechanical properties of cells, based on the micropipette aspiration technique or on the cell transit through flow constrictions. We applied this method to measure the elastic parameters of lymphocytes, in which the mechanical properties depend on their activation state. Additionally, we discuss in this work the accuracy of previous models to estimate the elastic modulus of cells, in particular the analytical model by Theret et al., widely used in the field. We show the necessity of using an improved model, taking into account the finite size of the cells, to obtain new insights that may remain hidden otherwise.
细胞的机械变形能力对于其功能和发育而言是一项重要特性，同时也是细胞状态的一个有用标志物。经典的微吸管吸液技术可用于单细胞研究，在此我们提供一种测量两个基本力学参数——弹性模量和泊松比的方法。所提出的方法是基于微吸管吸液实验的有限元分析而开发的，未来可基于微吸管吸液技术或细胞通过流动狭窄处的情况，应用于细胞力学性质自动测量的技术中。我们应用该方法测量淋巴细胞的弹性参数，淋巴细胞的力学性质取决于其激活状态。此外，我们在这项工作中讨论了先前模型估算细胞弹性模量的准确性，特别是Theret等人在该领域广泛使用的解析模型。我们表明，有必要使用一个考虑细胞有限大小的改进模型，以获得否则可能会被忽略的新见解。
