Xu Lin, Shi Jianfeng, Li Caixia, Zhu Shengan, Li Bo
Key Laboratory of Western China's Resources and Environment Chemistry, Hexi University, Zhangye, 734000, Gansu, China.
Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China.
Antonie Van Leeuwenhoek. 2017 Apr;110(4):479-488. doi: 10.1007/s10482-016-0817-z. Epub 2017 Jan 17.
A strain 5-1-2 was isolated from a root nodule of Hedysarum multijugum collected from Zhangye city, Gansu province, north-west China. Phylogenetic analysis based on the 16S rRNA gene sequence and other housekeeping genes (recA and atpD) indicated that the strain represents a novel species in the genus Rhizobium close to the strain Rhizobium subbaraonis JC85 with similarities of 98.27, 88.92 and 89.62%, respectively. Strain 5-1-2 contained Q-10 as the predominant ubiquinone. Our results showed that the major fatty acids were feature 8 (C ω7c and/or C ω6c; 38.90%). In addition, the DNA-DNA hybridizations with the type strains R. subbaraonis JC85 and Rhizobium halophytocola YC6881 were 39.2 ± 2.1 and 44.3 ± 1.9, respectively. Therefore, a novel species Rhizobium hedysari sp. nov. is proposed, and 5-1-2 (=CGMCC1.15677 = NBRC112532 is designated as the type strain.
从中国西北甘肃省张掖市采集的多叶岩黄芪根瘤中分离出菌株5-1-2。基于16S rRNA基因序列和其他看家基因（recA和atpD）的系统发育分析表明，该菌株代表根瘤菌属中的一个新物种，与根瘤菌Subbaraonis JC85菌株亲缘关系较近，相似性分别为98.27%、88.92%和89.62%。菌株5-1-2以泛醌Q-10作为主要泛醌。我们的结果表明，主要脂肪酸为特征8（C ω7c和/或C ω6c；38.90%）。此外，与模式菌株Subbaraonis JC85和盐生植物根瘤菌YC6881的DNA-DNA杂交率分别为39.2 ± 2.1和44.3 ± 1.9。因此，提出了一个新物种——多叶岩黄芪根瘤菌（Rhizobium hedysari sp. nov.），并将5-1-2（=CGMCC1.15677 = NBRC112532）指定为模式菌株。
