Xu Lin, Zhang Yong, Read Nicholas, Liu Shuangjiang, Friman Ville-Petri
Key Laboratory of Hexi Corridor Resources Utilization, Hexi University, Zhangye, 734000, Gansu, China.
Institute of Agricultural and Biological Technology, Hexi University, Zhangye, 734000, Gansu, China.
Antonie Van Leeuwenhoek. 2017 Nov;110(11):1475-1483. doi: 10.1007/s10482-017-0901-z. Epub 2017 Jun 24.
An aerobic, Gram-stain negative, short rod-shaped and motile strain, 36-5-1, was isolated from the roots of Nitraria sibirica in Zhangye city, Gansu province, north-west of China. Phylogenetic analysis based on the 16S rRNA gene sequence and two housekeeping genes (glnA and atpD) indicated that the strain represents a novel species closely related to the Devosia, Rhizobium and Devosia genera with 98.3, 96.2 and 91.1% similarities, respectively. The strain 36-5-1 contained Q-10 as the predominant ubiquinone and 16:0 (36.8%) as the major fatty acid; a large amount of unidentified glycolipid, diphosphatidylglycerol, phosphatidylglycerol and a small amount of unidentified polar lipids were present as polar lipids. In addition, the G+C content of the genomic DNA was 61.7 mol% and the DNA-DNA hybridization with type strains Devosia geojensis BD-c194 and Devosia pacifica NH131 44.1 ± 1.1 and 40.2 ± 1.7, respectively. Based on chemotaxonomic data and molecular properties, strain 36-5-1 represents a novel species within the genus Devosia, for which the name Devosia nitraria sp. nov. is proposed. The type strain is 36-5-1 (=CGMCC1.15704=NBRC112416.
从中国西北部甘肃省张掖市的西伯利亚白刺根部分离出一株需氧、革兰氏染色阴性、短杆状且具运动性的菌株36-5-1。基于16S rRNA基因序列及两个管家基因（glnA和atpD）的系统发育分析表明，该菌株代表一个新物种，与德沃斯氏菌属、根瘤菌属和德沃斯氏菌属密切相关，相似性分别为98.3%、96.2%和91.1%。菌株36-5-1以Q-10作为主要泛醌，以16:0（36.8%）作为主要脂肪酸；作为极性脂类，存在大量未鉴定的糖脂、双磷脂酰甘油、磷脂酰甘油以及少量未鉴定的极性脂类。此外，基因组DNA的G+C含量为61.7 mol%，与模式菌株德沃斯氏土生菌BD-c194和太平洋德沃斯氏菌NH131的DNA-DNA杂交率分别为44.1±1.1和40.2±1.7。基于化学分类数据和分子特性，菌株36-5-1代表德沃斯氏菌属内的一个新物种，为此提出新名称德沃斯氏西伯利亚白刺菌（Devosia nitraria sp. nov.）。模式菌株为36-5-1（=CGMCC1.15704=NBRC112416）。
