Department of Catalysis and Chemical Reaction Engineering, National Institute of Chemistry, Hajdrihova 19, 1001 Ljubljana, Slovenia.
Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695-8204, USA.
Int J Mol Sci. 2024 Aug 21;25(16):9044. doi: 10.3390/ijms25169044.
The natural aromatic polymer lignin and its lignin-like oligomeric fragments have attracted attention for their antioxidant capacity and free radical scavenging activities. In this study, a 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was employed to assess the antioxidant capacity of fractionated and partially depolymerized organosolv lignin by electron paramagnetic resonance (EPR) and UV-Vis spectroscopy. The results show significant antioxidant activity for both the lignin and oligomeric fragments, with the EPR measurements demonstrating their efficiency in quenching the free radicals. The EPR data were analyzed to derive the kinetic rate constants. The radical scavenging activity (RSA) of lignins was then determined by UV-Vis spectroscopy and the results were compared with the EPR method. This two-method approach improves the reliability and understanding of the antioxidant potential of lignin and its derivatives and provides valuable insights for their potential applications in various industries, including pharmaceuticals, food preservation, and cosmetics.
天然芳香聚合物木质素及其木质素样低聚物片段因其抗氧化能力和自由基清除活性而受到关注。在这项研究中，采用 2,2-二苯基-1-苦基肼（DPPH）法通过电子顺磁共振（EPR）和紫外可见光谱法评估分级和部分解聚的有机溶剂木质素的抗氧化能力。结果表明，木质素和低聚物片段均具有显著的抗氧化活性，EPR 测量表明它们能够有效淬灭自由基。对 EPR 数据进行了分析以得出动力学速率常数。然后通过紫外可见光谱法测定木质素的自由基清除活性（RSA），并将结果与 EPR 方法进行比较。这种双方法方法提高了木质素及其衍生物抗氧化潜力的可靠性和理解，并为它们在制药、食品保鲜和化妆品等各个行业的潜在应用提供了有价值的见解。
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