Liu Xin, Wu Xin, Ma Yuefan, Zhang Wenzhang, Hu Liang, Feng Xiaowei, Li Xiangyong, Tang Xudong
Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Xiashan, Zhanjiang, Guangdong 524023, P.R. China.
Oncol Rep. 2017 Mar;37(3):1793-1803. doi: 10.3892/or.2017.5366. Epub 2017 Jan 16.
The secondary metabolites of mangrove-derived endophytic fungi contain multiple substances with novel structures and biological activities. In the present study, three types of mangrove plants, namely Kandelia candel, Rhizophora stylosa and Rhizophoraceae from Zhanjiang region including the leaves, roots and stems were collected, and endophytic fungi were isolated, purified and identified from these mangrove plants. MTT assay was used to observe the effects of the isolated endophytic fungi on the growth of A549 and NCI-H460 lung cancer cells. The effect of the endophytic fungi on lung cancer angiogenesis in vitro induced by the HPV-16 E7 oncoprotein was observed. Our results showed that 28 strains of endophytic fungi were isolated, purified and identified from the three types of mangrove plants. Ten strains of endophytic fungi significantly suppressed the growth of A549 and NCI-H460 cells. The average inhibitory rates in the A549 cells were 64.4, 59.5, 81.9, 43.9, 58.3, 56.2, 48.3, 42.4, 93.0 and 49.7%, respectively. The average inhibitory rates in the NCI-H460 cells were 41.2, 49.3, 82.7, 40.7, 53.9, 52.6, 56.8, 64.3, 91.0 and 45.6%, respectively. Particularly, three strains of endophytic fungi markedly inhibited HPV-16 E7 oncoprotein‑induced lung cancer angiogenesis in vitro. These findings contribute to the further screening of potential chemotherapeutic agents from mangrove-derived endophytic fungi.
红树林内生真菌的次生代谢产物含有多种具有新颖结构和生物活性的物质。在本研究中，采集了湛江地区的三种红树林植物，即秋茄、红海榄和红树科植物的叶、根和茎，从这些红树林植物中分离、纯化并鉴定内生真菌。采用MTT法观察分离得到的内生真菌对A549和NCI-H460肺癌细胞生长的影响。观察了内生真菌对HPV-16 E7癌蛋白体外诱导的肺癌血管生成的影响。我们的结果表明，从三种红树林植物中分离、纯化并鉴定出28株内生真菌。10株内生真菌显著抑制A549和NCI-H460细胞的生长。在A549细胞中的平均抑制率分别为64.4%、59.5%、81.9%、43.9%、58.3%、56.2%、48.3%、42.4%、93.0%和49.7%。在NCI-H460细胞中的平均抑制率分别为41.2%、49.3%、82.7%、40.7%、53.9%、52.6%、56.8%、64.3%、91.0%和45.6%。特别地，三株内生真菌显著抑制HPV-16 E7癌蛋白体外诱导的肺癌血管生成。这些发现有助于从红树林内生真菌中进一步筛选潜在的化疗药物。
