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叶片及五种相关内生真菌乙酸乙酯提取物的体外抗氧化和抗菌活性

In Vitro Antioxidant and Antibacterial Activities of Ethyl Acetate Extracts of Leaves and Five Associated Endophytic Fungi.

作者信息

Ghazi-Yaker Amel, Kraak Bart, Houbraken Jos, Nabti El-Hafid, Cruz Cristina, Saadoun Noria, Houali Karim

机构信息

Natural Resources Laboratory, Faculty of Biological and Agronomic Sciences, Mouloud Mammeri Univesity, Tizi-Ouzou 15000, Algeria.

Laboratory of Analytic Biochemistry and Biotechnology (LABAB), Department of Biochemistry and Microbiology, Faculty of Biological and Agronomic Sciences, Mouloud Mammeri University, Tizi-Ouzou 15000, Algeria.

出版信息

Microorganisms. 2024 Dec 23;12(12):2671. doi: 10.3390/microorganisms12122671.

DOI:10.3390/microorganisms12122671
PMID:39770873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11728511/
Abstract

The exploration of new pharmacological compounds from endophytic fungi offers infinite possibilities. The aim of this study was to evaluate the antibacterial and antioxidant activities of extracts from the leaves of and five of its endophytic fungi and investigate the chemical diversity of the secondary metabolites produced. Isolated, purified, and molecularly identified endophytes and plant leaves were subjected to ethyl acetate extraction. The antibacterial potential of the extracts was assessed by the disc diffusion method against five bacterial strains: ATCC 25923; MU50; WDCM00009; ATCC 25922; and ATCC 27853. DPPH and reducing power tests were performed to assess antioxidant potential. GC-MS analysis was used to identify volatile compounds in extracts. Fungal endophytes were identified as , , , and , with respective accession numbers DTO 412-G6, DTO 412-I5, DTO 413-E7, DTO 412-G4, and DTO 414-I2. GC-MS analysis revealed a large number of bioactive compounds. All extracts showed antibacterial activity against at least two of the bacteria tested, and most showed antioxidant activity. The extract stood out for its higher phenolic content and higher antioxidant and antibacterial activities in all tests.

摘要

从内生真菌中探索新的药理化合物提供了无限可能。本研究的目的是评估[植物名称]叶片及其五种内生真菌提取物的抗菌和抗氧化活性，并研究所产生次生代谢物的化学多样性。对分离、纯化并经分子鉴定的内生菌和植物叶片进行乙酸乙酯萃取。通过纸片扩散法评估提取物对五种细菌菌株的抗菌潜力：金黄色葡萄球菌ATCC 25923；耐甲氧西林金黄色葡萄球菌MU50；肺炎克雷伯菌WDCM00009；大肠埃希菌ATCC 25922；以及铜绿假单胞菌ATCC 27853。进行DPPH和还原力测试以评估抗氧化潜力。采用气相色谱 - 质谱联用（GC-MS）分析来鉴定提取物中的挥发性化合物。真菌内生菌被鉴定为[具体真菌名称1]、[具体真菌名称2]、[具体真菌名称3]、[具体真菌名称4]和[具体真菌名称5]，各自的登录号分别为DTO 412 - G6、DTO 412 - I5、DTO 413 - E7、DTO 412 - G4和DTO 414 - I2。GC-MS分析揭示了大量生物活性化合物。所有提取物对至少两种测试细菌表现出抗菌活性，并且大多数表现出抗氧化活性。[植物名称]提取物在所有测试中因其较高的酚类含量以及较高的抗氧化和抗菌活性而脱颖而出。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/724723fb1123/microorganisms-12-02671-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/523561444171/microorganisms-12-02671-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/b2db5eba0845/microorganisms-12-02671-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/d10082f18395/microorganisms-12-02671-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/724723fb1123/microorganisms-12-02671-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/523561444171/microorganisms-12-02671-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/b2db5eba0845/microorganisms-12-02671-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/d10082f18395/microorganisms-12-02671-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b334/11728511/724723fb1123/microorganisms-12-02671-g004.jpg

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