Guerrero Abraham, Lizárraga-Partida Marcial Leonardo, Gómez Gil Rodríguez Bruno, Licea-Navarro Alexei Fedorovish, Revilla-Castellanos Valeria Jeanette, Wong-Chang Irma, González-Sánchez Ricardo
Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California, (CICESE), Ensenada, Baja California, México.
Mazatlan Unit for Aquaculture and Environmental Management, (CIAD, AC), Mazatlán, Sinaloa, México.
PLoS One. 2017 Jan 18;12(1):e0169722. doi: 10.1371/journal.pone.0169722. eCollection 2017.
Vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. Environmental and clinical toxigenic strains of V. parahaemolyticus that were isolated in Mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the O3:K6 pandemic clone, and their genetic relationship to strains that are related to the pandemic clonal complex (CC3). Pathogenic tdh+ and tdh+/trh+ strains were analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Also, the entire genome of a Mexican O3:K6 strain was sequenced. Most of the strains were tdh/ORF8-positive and corresponded to the O3:K6 serotype. By PFGE and MLST, there was very close genetic relationship between ORF8/O3:K6 strains, and very high genetic diversities from non-pandemic strains. The genetic relationship is very close among O3:K6 strains that were isolated in Mexico and sequences that were available for strains in the CC3, based on the PubMLST database. The whole-genome sequence of CICESE-170 strain had high similarity with that of the reference RIMD 2210633 strain, and harbored 7 pathogenicity islands, including the 4 that denote O3:K6 pandemic strains. These results indicate that pandemic strains that have been isolated in Mexico show very close genetic relationship among them and with those isolated worldwide.
副溶血性弧菌是一种重要的人类病原体，已在全球范围内从临床病例中分离出来，其中大多数与食用海鲜有关。对1998年至2012年在墨西哥分离出的副溶血性弧菌的环境和临床产毒株，包括该国唯一报告的疫情中的菌株，进行了基因特征分析，以评估O3:K6大流行克隆的存在，以及它们与大流行克隆复合体（CC3）相关菌株的遗传关系。通过脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）对致病性tdh+和tdh+/trh+菌株进行了分析。此外，还对一株墨西哥O3:K6菌株的全基因组进行了测序。大多数菌株tdh/ORF8呈阳性，对应于O3:K6血清型。通过PFGE和MLST分析，ORF8/O3:K6菌株之间存在非常密切的遗传关系，与非大流行菌株的遗传多样性非常高。根据PubMLST数据库，墨西哥分离的O3:K6菌株与CC3中可用菌株序列之间的遗传关系非常密切。CICESE-170菌株的全基因组序列与参考菌株RIMD 2210633具有高度相似性，并含有7个致病岛，包括4个表示O3:K6大流行菌株的致病岛。这些结果表明，在墨西哥分离出的大流行菌株之间以及与全球分离出的菌株之间显示出非常密切的遗传关系。
