Cátedras CONACyT-CIAD, Food Research and Development Center A.C. Mazatlán Unit (Centro de Investigación en Alimentación y Desarrollo, A.C. Unidad Mazatlán), Mazatlán, Sinaloa, Mexico.
CIAD, Food Research and Development Center A.C. Mazatlán Unit for Aquaculture, A.P. 711, Mazatlán, Sinaloa, Mexico, 82100.
BMC Genom Data. 2021 Sep 27;22(1):38. doi: 10.1186/s12863-021-00985-0.
The V. parahaemolyticus pandemic clone, results in the development of gastrointestinal illness in humans. Toxigenic strains of this species are frequently isolated from aquatic habitats and organisms such as mollusks and crustaceans. Reports on the isolation of the pandemic clone started in 1996, when a new O3:K6 clone was identified in Asia, that rapidly spread worldwide, becoming the predominant clone isolated from clinical cases. In this study whole genome sequencing was accomplished with an Illumina MiniSeq platform, upon six novel V. parahaemolyticus strains, that have been isolated in Mexico since 1998 and three representative genomes of strains that were isolated from reported outbreaks in other American countries, and were deposited in the GenBank. These nine genomes were compared against the reference sequence of the O3:K6 pandemic strain (RIMD 2210633), which was isolated in 1996, to determine sequence differences within American isolates and between years of isolation.
The results indicated that strains that were isolated at different times and from different countries, were highly genetically similar, among them as well as to the reference strain RIMD 2210633, indicating a high level of genetic stability among the strains from American countries between 1996 to 2012, without significant genetic changes relative to the reference strain RIMD 2210633, which was isolated in 1996 and was considered to be representative of a novel O3:K6 pandemic strain.
The genomes of V. parahaemolyticus strains isolated from clinical and environmental sources in Mexico and other American countries, presented common characteristics that have been reported for RIMD 2210633 O3:K6 pandemic strain. The major variations that were registered in this study corresponded to genes non associated to virulence factors, which could be the result of adaptations to different environmental conditions. Nevertheless, results do not show a clear pattern with the year or locality where the strains were isolated, which is an indication of a genomic stability of the studied strains.
副溶血性弧菌流行株可导致人类胃肠道疾病。该物种的产毒菌株常从水生栖息地和贝类、甲壳类等生物中分离出来。1996 年首次报告从亚洲分离到流行株 O3:K6 新克隆，此后该克隆迅速在全球范围内传播，成为从临床病例中分离到的主要克隆。本研究采用 Illumina MiniSeq 平台对 6 株于 1998 年以来在墨西哥分离的新型副溶血性弧菌菌株以及 3 株分别来自其他美洲国家报告暴发的代表株进行全基因组测序，并将其序列提交至 GenBank。将这 9 个基因组与 1996 年分离的 O3:K6 流行株参考序列（RIMD 2210633）进行比较，以确定美洲分离株之间以及不同分离年份之间的序列差异。
结果表明，不同时间和不同国家分离的菌株具有高度的遗传相似性，与参考菌株 RIMD 2210633 也高度相似，这表明 1996 年至 2012 年期间，来自美洲国家的菌株之间遗传稳定性较高，与 1996 年分离的代表新型 O3:K6 流行株的参考菌株 RIMD 2210633 相比，没有明显的遗传变化。
从墨西哥和其他美洲国家临床和环境来源分离的副溶血性弧菌菌株基因组具有与 RIMD 2210633 O3:K6 流行株报道的共同特征。本研究中登记的主要变异与毒力因子无关的基因有关，这可能是对不同环境条件的适应结果。然而，结果并没有显示出与菌株分离的年份或地点之间有明显的模式，这表明所研究的菌株基因组具有稳定性。
