Yang Ying, Xie Jiafang, Li Hua, Tan Shuwen, Chen Yanfeng, Yu Hui
School of Life Science and Engineering, Foshan University, Foshan, China.
Front Microbiol. 2017 Dec 20;8:2566. doi: 10.3389/fmicb.2017.02566. eCollection 2017.
is a leading cause of foodborne infections in China and a threat to human health worldwide. The main objective of this study is to determine the prevalence and characteristic of isolates in fish, oyster and shrimp samples from the South China domestic consumer market. To accomplish this, we examined 504 seafood samples from 11 provinces of China. The prevalence rates were 9.38, 30.36, and 25.60%, respectively. In summer (33.33%), the prevalence of was more common than that detected in the winter (14.01%). In addition, we identified 98 strains. The antimicrobial resistance trends of our seafood isolates to 15 antimicrobial agents revealed that major isolates were resistant to ampicillin (79.59%). Furthermore, 68.38% of the isolates were identified as being multidrug resistance. The prevalence of or genes among the isolates was 8.16 and 12.24%, respectively. ERIC-PCR and multilocus sequence typing (MLST) results enabled classification of the isolates ( = 98) into different clusters, revealing genetic variation and relatedness among the isolates. Thus, our findings demonstrate the prevalence of in a variety of common seafood consumed domestically in China and provides insights into the dissemination of antibiotic-resistant strains, which should improve our microbiological risk assessment knowledge associated with in seafoods.
是中国食源性感染的主要原因，也是全球对人类健康的一大威胁。本研究的主要目的是确定中国南方国内消费市场鱼类、牡蛎和虾类样本中分离株的流行情况和特征。为实现这一目标，我们检测了来自中国11个省份的504份海鲜样本。其流行率分别为9.38%、30.36%和25.60%。在夏季（33.33%），该菌的流行比冬季（14.01%）更为常见。此外，我们鉴定出98株该菌。我们的海鲜分离株对15种抗菌药物的耐药趋势表明，主要分离株对氨苄青霉素耐药（79.59%）。此外，68.38%的分离株被鉴定为多重耐药。分离株中该菌或该菌基因的流行率分别为8.16%和12.24%。ERIC-PCR和多位点序列分型（MLST）结果能够将分离株（n = 98）分为不同的簇，揭示了分离株之间的遗传变异和相关性。因此，我们的研究结果证明了该菌在中国国内消费的各种常见海鲜中的流行情况，并为抗生素耐药菌株的传播提供了见解，这将提高我们与海鲜中该菌相关的微生物风险评估知识。
