Stringel G, Uauy R, Guertin L
Department of Surgery, University of Texas Southwestern Medical Center, Dallas.
J Pediatr Surg. 1989 Oct;24(10):1086-8. doi: 10.1016/s0022-3468(89)80223-4.
We evaluated the effect of intestinal anastomosis without resection on gut morphometry (muscle thickness, villus height, and crypt depth), growth indices (DNA, protein, and protein:DNA ratio), and disaccharidase activity (maltase, sucrase, and lactase) in the growing animal. A group of 10 weanling Sprague-Dawley rats at 21 days of age was subjected to intestinal transection and anastomosis in the upper jejunum, 10 cm distal to the ligament of Treitz. A second group of 10 similar rats was used as a control group. All rats were fed a regular diet and kept under the same conditions. They were sacrificed 2 weeks later. Body weight, intestinal weight, and intestinal length measurements were obtained. The intestine was divided into two sections: preanastomotic (section A) and postanastomotic (section B) in the surgery group and equivalent sections A and B in the control group. Specimens were subjected to morphometric evaluation and mucosal scrapings for biochemical analysis. Despite significant weight gain in the control group, there were no differences in intestinal length, intestinal weight, and mucosal weight between the two groups. Muscle thickness, villus height, and crypt depth were significantly increased in the preanastomotic segment. Protein and DNA were also higher in the preanastomotic segment, but the protein:DNA ratio was less affected. There was significantly decreased enzymatic activity in the preanastomotic segment. Intestinal anastomosis has a significant effect on gut growth and maturation in the growing animal and may have important implications in the postoperative management of newborns and infants following intestinal surgery.
我们评估了不进行切除的肠道吻合术对生长中动物的肠道形态计量学（肌肉厚度、绒毛高度和隐窝深度）、生长指标（DNA、蛋白质和蛋白质:DNA比率）以及双糖酶活性（麦芽糖酶、蔗糖酶和乳糖酶）的影响。选取一组10只21日龄的断奶斯普拉格-道利大鼠，在距屈氏韧带远端10厘米处的空肠上段进行肠横断和吻合术。另一组10只相似的大鼠作为对照组。所有大鼠均喂食常规饮食，并饲养在相同条件下。2周后将它们处死，测量体重、肠道重量和肠道长度。手术组的肠道分为两段：吻合术前段（A段）和吻合术后段（B段），对照组则分为等效的A段和B段。对标本进行形态计量学评估，并刮取黏膜进行生化分析。尽管对照组体重显著增加，但两组之间的肠道长度、肠道重量和黏膜重量并无差异。吻合术前段的肌肉厚度、绒毛高度和隐窝深度显著增加。吻合术前段的蛋白质和DNA含量也较高，但蛋白质:DNA比率受影响较小。吻合术前段的酶活性显著降低。肠道吻合术对生长中动物的肠道生长和成熟有显著影响，可能对新生儿和婴儿肠道手术后的术后管理具有重要意义。
