Goverse Gera, Molenaar Rosalie, Macia Laurence, Tan Jian, Erkelens Martje N, Konijn Tanja, Knippenberg Marlene, Cook Emma C L, Hanekamp Diana, Veldhoen Marc, Hartog Anita, Roeselers Guus, Mackay Charles R, Mebius Reina E
Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 HZ Amsterdam, the Netherlands.
Department of Immunology, Monash University, Clayton, Victoria 3800, Australia.
J Immunol. 2017 Mar 1;198(5):2172-2181. doi: 10.4049/jimmunol.1600165. Epub 2017 Jan 18.
The gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. In this article, we demonstrate that dietary fiber and short chain fatty acids (SCFAs) induced the expression of the vitamin A-converting enzyme RALDH1 in intestinal epithelial cells in vivo and in vitro, respectively. Furthermore, our data showed that the expression levels of RALDH1 in small intestinal epithelial cells correlated with the activity of vitamin A-converting enzymes in mesenteric lymph node dendritic cells, along with increased numbers of intestinal regulatory T cells and a higher production of luminal IgA. Moreover, we show that the consumption of dietary fiber can alter the composition of SCFA-producing microbiota and SCFA production in the small intestines. In conclusion, our data illustrate that dietary adjustments affect small intestinal epithelial cells and can be used to modulate the mucosal immune system.
胃肠道持续暴露于许多影响肠上皮细胞和潜在黏膜免疫系统的环境因素中。在本文中，我们分别证明了膳食纤维和短链脂肪酸（SCFAs）在体内和体外诱导肠上皮细胞中维生素A转化酶RALDH1的表达。此外，我们的数据表明，小肠上皮细胞中RALDH1的表达水平与肠系膜淋巴结树突状细胞中维生素A转化酶的活性相关，同时肠道调节性T细胞数量增加，管腔IgA产生增多。而且，我们表明膳食纤维的摄入可改变小肠中产生SCFA的微生物群组成和SCFA的产生。总之，我们的数据表明饮食调整会影响小肠上皮细胞，并可用于调节黏膜免疫系统。
