Zhang Hong, Li Haiqing, Liu Yan, Li Qingyan, Bi Yufang, Fang Guiqing
Department of Clinical Laboratory, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China.
Department of Nursing, The Sixth People's Hospital of Jinan, Jinan, Shandong 250200, P.R. China.
Exp Ther Med. 2016 Dec;12(6):3571-3574. doi: 10.3892/etm.2016.3805. Epub 2016 Oct 14.
The aim of the study was to investigate the characteristic function of the upregulated effects of miR-7 in methicillin-resistant (MRSA). After separating the MRSA in clinic, the expression of miR-7 mRNA was tested by reverse transcription polymerase chain reaction. The overexpression, inhibition of miR-7, and control group were established by plasmid . Following transfection of the bacterial strain, the effect of β-lactam antibiotics in minimum inhibitory concentration (MIC) was observed using the microporous dilution method, and antibacterial effects were observed using the dynamic growth curve method. The expression of miR-7 in sensitive MRSA was upregulated distinctly, with significant difference (P<0.05). MIC and the number of bacteria in the miR-7 overexpression group significantly increased while the inhibition group decreased prominently, with significant difference (P<0.05). The control and null plasmid groups revealed no significant difference. In conclusion, miR-7 upregulated the antimicrobial activity of MRSA, and the intervention of its expression may become a possible antibacterial target.
本研究旨在探讨miR-7在耐甲氧西林金黄色葡萄球菌（MRSA）中上调作用的特征功能。临床分离出MRSA后，采用逆转录聚合酶链反应检测miR-7 mRNA的表达。通过质粒建立miR-7的过表达组、抑制组和对照组。菌株转染后，采用微孔稀释法观察β-内酰胺类抗生素在最低抑菌浓度（MIC）方面的效果，并采用动态生长曲线法观察抗菌效果。敏感MRSA中miR-7的表达明显上调，差异有统计学意义（P<0.05）。miR-7过表达组的MIC和细菌数量显著增加，而抑制组则显著减少，差异有统计学意义（P<0.05）。对照组和空质粒组无显著差异。综上所述，miR-7上调了MRSA的抗菌活性，对其表达的干预可能成为一个潜在的抗菌靶点。
