Lin Bin, Yu Hui, He Yongzhi, Xu Yang, Zhang Wenbin, Lu Chengwu, Ao Qingfang
Department of Orthopaedics, The 175th Hospital of PLA, Southeast Hospital of Xiamen University, Zhangzhou, Fujian 363000, P.R. China.
Exp Ther Med. 2016 Dec;12(6):3917-3922. doi: 10.3892/etm.2016.3878. Epub 2016 Nov 7.
Nucleus pulposus (NP) has been suggested to trigger an autoimmune response if exposed to the immune system, which plays a key role in neuropathic pain. Therefore, appropriate suppression of inflammation is a key factor for treating the radiculopathy caused by intervertebral disk (IVD) degeneration. Resveratrol, a key component of red wine, has been suggested to exhibit anti-inflammatory properties and . However, the effects of resveratrol on NP-mediated pain have not been studied. The aim of the present study was to investigate whether resveratrol may be useful in treating NP-mediated pain in an autologous NP model of radiculopathy. A total of 36 adult male Sprague-Dawley rats were allocated randomly into sham (group I), saline-treated (group II) and resveratrol-treated (group III) groups. Animal behavior in response to non-noxious mechanical stimulation with von Frey filaments was compared at days 0 (baseline), 3, 7, 14 and 21 following surgery. The expression of proinflammatory cytokines such as tumor necrosis factor α (TNF-α) and interleukin-1 (IL-1) were assessed at days 7 and 14. The data showed that resveratrol exhibited an anti-inflammatory effect on the expression of proinflammatory cytokines. Compared with group II, the expression of TNF-α and IL-1 was significantly decreased at each time point in group III. In addition, resveratrol significantly reduced pain behavior triggered by the application of NP tissue on the dorsal root ganglion for up to 14 days. These data suggest that resveratrol has potential for the treatment of NP-mediated pain, indicating a potential clinical application.
有研究表明，如果髓核（NP）暴露于免疫系统，会引发自身免疫反应，这在神经性疼痛中起关键作用。因此，适当抑制炎症是治疗椎间盘（IVD）退变引起的神经根病的关键因素。白藜芦醇是红酒的关键成分，已被证明具有抗炎特性。然而，白藜芦醇对NP介导的疼痛的影响尚未得到研究。本研究的目的是调查白藜芦醇在自体NP模型神经根病中是否可用于治疗NP介导的疼痛。总共36只成年雄性Sprague-Dawley大鼠被随机分为假手术组（I组）、生理盐水治疗组（II组）和白藜芦醇治疗组（III组）。在术后第0天（基线）、3天、7天、14天和21天，比较动物对用von Frey细丝进行的非伤害性机械刺激的行为反应。在第7天和14天评估促炎细胞因子如肿瘤坏死因子α（TNF-α）和白细胞介素-1（IL-1）的表达。数据显示，白藜芦醇对促炎细胞因子的表达具有抗炎作用。与II组相比，III组在每个时间点TNF-α和IL-1的表达均显著降低。此外，白藜芦醇在长达14天的时间内显著减轻了将NP组织应用于背根神经节引发的疼痛行为。这些数据表明，白藜芦醇在治疗NP介导的疼痛方面具有潜力，显示出潜在的临床应用价值。
