Ferreira-Rodrigues Sára Cósta, Rodrigues Cássio Milhomens, Dos Santos Marcio Galdino, Gautuz Jean Antonio Abraham, Silva Magali Glauzer, Cogo José Carlos, Batista-Silva Camila, Dos Santos Cleiton Pita, Groppo Francisco Carlos, Cogo-Müller Karina, Oshima-Franco Yoko
Environmental Sciences Post-Graduate Program, PGCiamb, Tocantins Federal University (UFT), Palmas, Tocantins, Brazil.
Post-Graduate Program in Pharmaceutical Sciences and Pharmacy, University of Sorocaba (UNISO), Sorocaba, SP, Brazil.
Adv Pharm Bull. 2016 Dec;6(4):573-579. doi: 10.15171/apb.2016.071. Epub 2016 Dec 22.
The aim of this study was to evaluate the antibothropic and anti-inflammatory properties of Phytochemical screening and thin-layer chromatography (TLC) assays were performed on hydroalcoholic extract (TE) in order to observe its main constituents. The antibothropic activity of TE was evaluated by the neuromuscular blockade caused by venom (Bjssu), in a mouse phrenic nerve-diaphragm model (PND). A quantitative histological study was carried out to observe a possible protection of TE against the venom myotoxicity. The anti-inflammatory activity was also evaluated in two models, Bjssu-induced paw edema, and carrageenan-induced neutrophils migration in the peritoneal cavity. TLC analysis revealed several compounds in TE, such as saponins, alkaloids, and phenolic constituents. TE was able to neutralize the blockade and the myotoxicity induced by venom, when it was pre-incubated for 30 min with venom. In addition, it showed anti-inflammatory activity, inducing less neutrophils migration and reducing paw edema. showed both antibothropic and anti-inflammatory properties.
本研究的目的是评估[提取物名称未明确]的抗蛇毒和抗炎特性。对水醇提取物（TE）进行了植物化学筛选和薄层色谱（TLC）分析，以观察其主要成分。在小鼠膈神经 - 膈肌模型（PND）中，通过毒液（Bjssu）引起的神经肌肉阻滞来评估TE的抗蛇毒活性。进行了定量组织学研究，以观察TE对毒液肌毒性的可能保护作用。还在两种模型中评估了抗炎活性，即Bjssu诱导的爪肿胀和角叉菜胶诱导的腹腔中性粒细胞迁移。TLC分析显示TE中有几种化合物，如皂苷、生物碱和酚类成分。当TE与毒液预孵育30分钟时，它能够中和毒液诱导的阻滞和肌毒性。此外，它还表现出抗炎活性，减少中性粒细胞迁移并减轻爪肿胀。[提取物名称未明确]显示出抗蛇毒和抗炎特性。
