Wolf Martin, Halper Maria, Pribyl Raffaela, Baurecht Dieter, Valenta Claudia
University of Vienna, Research platform 'Characterisation of Drug Delivery Systems on Skin and Investigations of Involved Mechanisms', Althanstraße 14, 1090 Vienna, Austria.
University of Vienna, Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Life Sciences, Althanstraße 14, 1090 Vienna, Austria.
Int J Pharm. 2017 Mar 15;519(1-2):198-205. doi: 10.1016/j.ijpharm.2017.01.026. Epub 2017 Jan 17.
The spatial distribution of exogenous substances in the stratum corneum (SC) could have an influence on their skin irritation potential. In this study it was possible to monitor the distribution of phospholipids with their phosphatidylcholine scaffold on porcine ear skin by combining tape stripping and in vitro ATR-FTIR spectroscopy. Significant vibrational modes in the spectra could be successfully assigned to the functional groups of the molecules. Thus it was possible to track the phospholipids without the need of their deuterated form by calculating difference spectra from the treated - untreated skin samples. The correlation between four characteristic bands (R≥0.9909) revealed the excellent suitability of this semi-quantitative method for deep profiling analysis. The penetration capabilities of aqueous suspensions of the different phospholipid compositions as well as two monoacyl-phosphatidylcholine based liposome formulations were investigated using this method. Nevertheless, differences in the distribution of the investigated phospholipid species, having different amounts of monoacyl-phosphatidylcholine, could not be found. It could be clearly shown that the deepest skin penetration was seen in the irritating anionic SDS (sodium dodecyl sulfate) out of the aqueous solution. The aqueous suspensions based on different phospholipid surfactants showed the same range of penetration depth (10-15% of SC), whereas the smallest skin penetration depth was observed after the application of liposomal formulations.
角质层（SC）中外源性物质的空间分布可能会影响其皮肤刺激潜能。在本研究中，通过结合胶带剥离和体外衰减全反射傅里叶变换红外光谱（ATR-FTIR），能够监测猪耳皮肤中带有磷脂酰胆碱支架的磷脂分布。光谱中的显著振动模式能够成功地与分子的官能团对应。因此，通过计算处理过的皮肤样本与未处理的皮肤样本的差异光谱，无需使用氘代形式就能追踪磷脂。四个特征波段之间的相关性（R≥0.9909）表明这种半定量方法非常适合深度剖析分析。使用该方法研究了不同磷脂组成的水悬浮液以及两种基于单酰基磷脂酰胆碱的脂质体制剂的渗透能力。然而，未发现所研究的具有不同单酰基磷脂酰胆碱含量的磷脂种类在分布上存在差异。可以清楚地表明，从水溶液中观察到，具有刺激性的阴离子型十二烷基硫酸钠（SDS）对皮肤的渗透最深。基于不同磷脂表面活性剂的水悬浮液显示出相同范围的渗透深度（占角质层的10 - 15%），而脂质体制剂应用后观察到的皮肤渗透深度最小。
