Krombholz Richard, Liu Yali, Lunter Dominique Jasmin
Department of Pharmaceutical Technology, Eberhard Karls University, Auf der Morgenstelle 8, 72076 Tuebingen, Germany.
Pharmaceutics. 2021 Jan 7;13(1):67. doi: 10.3390/pharmaceutics13010067.
Ex-vivo and in-vivo skin analysis has been extensively evaluated by confocal Raman spectroscopy (CRS). The off-line measurement with a CRS-suited skin-mounted device after Franz-cell incubations is the most popular choice. However, real-time monitoring of in-line measurement has clear advantages for obtaining dynamic and more timely results. In our study, a custom-built setup suitable for in-line measurements was implemented, which ensures constant skin incubation and in-situ skin detections. We aim to compare the differences between using in-line and off-line devices for monitoring skin drug penetrations. A well-assessed formulation gel with procaine-HCl as the active ingredient was used as reference. The PEG-23 lauryl ether was added to the formulation as a penetration enhancer to evaluate the enhancement effects of procaine on skin. After incubation times of 14, 20, and 24 h, skin penetration profiles were assessed. Comparable results between off-line and in-line measurements were obtained. Remarkable improvements in penetrated procaine amount and depth were observed. Based on the significant differences of their enhanced penetration amounts, fairly similar estimations were achieved from both methods. A slight difference of 14 h incubation between these two setups can still be found, which may be due to the different detection conditions and affected skin properties. Overall, in-line measurements could provide a more time- and labor-saving alternative for off-line measurements in ex-vivo study.
共聚焦拉曼光谱（CRS）已广泛用于体外和体内皮肤分析。在Franz扩散池孵育后，使用适合CRS的皮肤安装装置进行离线测量是最常用的方法。然而，在线测量的实时监测在获取动态和更及时的结果方面具有明显优势。在我们的研究中，实现了一种适合在线测量的定制装置，该装置可确保皮肤持续孵育和原位皮肤检测。我们旨在比较使用在线和离线装置监测皮肤药物渗透的差异。以盐酸普鲁卡因作为活性成分的一种经过充分评估的凝胶制剂用作参考。将聚乙二醇-23月桂醚添加到制剂中作为渗透促进剂，以评估普鲁卡因对皮肤的增强作用。在孵育14、20和24小时后，评估皮肤渗透情况。离线和在线测量获得了可比的结果。观察到渗透的普鲁卡因量和深度有显著改善。基于它们增强渗透量的显著差异，两种方法得到了相当相似的估计结果。这两种装置在孵育14小时时仍存在细微差异，这可能是由于检测条件不同以及皮肤性质受到影响。总体而言，在体外研究中，在线测量可为离线测量提供一种更省时省力的替代方法。
