Belabbes Rania, Dib Mohammed El Amine, Djabou Nassim, Ilias Faiza, Tabti Boufeldja, Costa Jean, Muselli Alain
Laboratoire des Substances Naturelles et Bioactives (LASNABIO), Université de Tlemcen, BP 119, 13000, Algéria.
Département de Chimie, Faculté des Sciences, Université Abou Bekr Belkaïd, BP 119, Tlemcen, 13000, Algéria.
Chem Biodivers. 2017 May;14(5). doi: 10.1002/cbdv.201600482. Epub 2017 May 3.
The chemical composition of the essential oils and hydrosol extract from aerial parts of Calendula arvensis L. was investigated using GC-FID and GC/MS. Intra-species variations of the chemical compositions of essential oils from 18 Algerian sample locations were investigated using statistical analysis. Chemical analysis allowed the identification of 53 compounds amounting to 92.3 - 98.5% with yields varied of 0.09 - 0.36% and the main compounds were zingiberenol 1 (8.7 - 29.8%), eremoligenol (4.2 - 12.5%), β-curcumene (2.1 - 12.5%), zingiberenol 2 (4.6 - 19.8%) and (E,Z)-farnesol (3.5 - 23.4%). The study of the chemical variability of essential oils allowed the discrimination of two main clusters confirming that there is a relation between the essential oil compositions and the harvest locations. Different concentrations of essential oil and hydrosol extract were prepared and their antioxidant activity were assessed using three methods (2,2-diphenyl-1-picrylhydrazyl, Ferric-Reducing Antioxidant Power Assay and β-carotene). The results showed that hydrosol extract presented an interesting antioxidant activity. The in vitro antifungal activity of hydrosol extract produced the best antifungal inhibition against Penicillium expansum and Aspergillus niger, while, essential oil was inhibitory at relatively higher concentrations. Results showed that the treatments of pear fruits with essential oil and hydrosol extract presented a very interesting protective activity on disease severity of pears caused by P. expansum.
采用气相色谱 - 火焰离子化检测器（GC - FID）和气相色谱 - 质谱联用仪（GC/MS）对田野金盏花地上部分的精油和水溶胶提取物的化学成分进行了研究。利用统计分析方法研究了来自18个阿尔及利亚采样地点的精油化学成分的种内变异。化学分析鉴定出53种化合物，含量为92.3 - 98.5%，产率在0.09 - 0.36%之间，主要化合物为姜烯醇1（8.7 - 29.8%）、异佛尔酮醇（4.2 - 12.5%）、β - 姜黄烯（2.1 - 12.5%）、姜烯醇2（4.6 - 19.8%）和（E,Z）- 法尼醇（3.5 - 23.4%）。对精油化学变异性的研究使得能够区分出两个主要聚类，证实了精油成分与收获地点之间存在关联。制备了不同浓度的精油和水溶胶提取物，并使用三种方法（2,2 - 二苯基 - 1 - 苦基肼、铁还原抗氧化能力测定法和β - 胡萝卜素）评估了它们的抗氧化活性。结果表明，水溶胶提取物具有显著的抗氧化活性。水溶胶提取物的体外抗真菌活性对扩展青霉和黑曲霉产生了最佳的抗真菌抑制作用，而精油在相对较高浓度下具有抑制作用。结果表明，用精油和水溶胶提取物处理梨果实对扩展青霉引起的梨病害严重程度具有非常显著的保护作用。
