Zhu Liqi, Yang Xing, Mou Chunxiao, Yang Qian
Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
Veterinary College, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
Vet Microbiol. 2017 Feb;199:128-134. doi: 10.1016/j.vetmic.2016.12.031. Epub 2016 Dec 21.
Coronaviruses tend to efficiently evade innate immune sensing. Alpha-coronaviruses interfere with the type I interferon (IFN) response in various ways, ensuring the limited activation of IFN responses. Transmissible gastroenteritis virus (TGEV), an Alphacoronavirus genera virus, is an important pathogen that mainly infects piglet, but little is known about the activation of the host immune response. We show that TGEV induces a delayed activation of the IFN response in intestinal epithelial cells. Briefly, IFN-β expression induced by TGEV infection is delayed with respect to that induced by poly(I:C) transfection. In addition, some of the IFN-stimulated genes (ISGs) were up-regulated in the early infection stage without obvious expression of IFN-β. Moreover, we show that activation of IFN responses induced by poly(I:C) could inhibit viral replication in the early infection stage, but failed in the late infection stage in IPEC-J2 cells. Finally, the activation of IFN responses induced by TGEV infection cannot inhibit viral replication. Taken together, this study provides a preliminary analysis of an interaction between TGEV and IFN-β responses of intestinal epithelial cells.
冠状病毒往往能够有效地逃避天然免疫感知。α冠状病毒以多种方式干扰I型干扰素（IFN）反应，确保IFN反应的激活受到限制。传染性胃肠炎病毒（TGEV）是一种α冠状病毒属病毒，是主要感染仔猪的重要病原体，但关于宿主免疫反应的激活情况知之甚少。我们发现，TGEV在肠道上皮细胞中诱导IFN反应的延迟激活。简而言之，TGEV感染诱导的IFN-β表达相对于聚肌苷酸胞苷酸（poly(I:C)）转染诱导的IFN-β表达延迟。此外，一些IFN刺激基因（ISGs）在感染早期上调，而IFN-β没有明显表达。此外，我们发现poly(I:C)诱导的IFN反应激活在感染早期可以抑制病毒复制，但在IPEC-J2细胞的感染后期则无效。最后，TGEV感染诱导的IFN反应激活不能抑制病毒复制。综上所述，本研究对TGEV与肠道上皮细胞IFN-β反应之间的相互作用进行了初步分析。
