Sedlar Karel, Kolek Jan, Provaznik Ivo, Patakova Petra
Department of Biomedical Engineering, Brno University of Technology, Technicka 12, 616 00 Brno, Czechia.
Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czechia.
J Biotechnol. 2017 Feb 20;244:1-3. doi: 10.1016/j.jbiotec.2017.01.003. Epub 2017 Jan 19.
The complete genome sequence of non-type strain Clostridium pasteurianum NRRL B-598 was introduced last year; it is an oxygen tolerant, spore-forming, mesophilic heterofermentative bacterium with high hydrogen production and acetone-butanol fermentation ability. The basic genome statistics have shown its similarity to C. beijerinckii rather than the C. pasteurianum species. Here, we present a comparative analysis of the strain with several other complete clostridial genome sequences. Besides a 16S rRNA gene sequence comparison, digital DNA-DNA hybridization (dDDH) and phylogenomic analysis confirmed an inaccuracy of the taxonomic status of strain Clostridium pasteurianum NRRL B-598. Therefore, we suggest its reclassification to be Clostridium beijerinckii NRRL B-598. This is a specific strain and is not identical to other C. beijerinckii strains. This misclassification explains its unexpected behavior, different from other C. pasteurianum strains; it also permits better understanding of the bacterium for a future genetic manipulation that might increase its biofuel production potential.
去年公布了非模式菌株巴斯德梭菌NRRL B - 598的全基因组序列；它是一种耐氧、产芽孢、嗜温的异型发酵细菌，具有高产氢和丙酮 - 丁醇发酵能力。基本的基因组统计数据显示它与拜氏梭菌的相似性高于巴斯德梭菌属。在此，我们对该菌株与其他几个完整的梭菌基因组序列进行了比较分析。除了16S rRNA基因序列比较外，数字DNA - DNA杂交（dDDH）和系统基因组分析证实了巴斯德梭菌NRRL B - 598菌株分类地位的不准确。因此，我们建议将其重新分类为拜氏梭菌NRRL B - 598。这是一个特定的菌株，与其他拜氏梭菌菌株不同。这种错误分类解释了它与其他巴斯德梭菌菌株不同的意外行为；这也有助于未来对该细菌进行遗传操作以提高其生物燃料生产潜力时能更好地了解它。
