Fuzer Angelina M, Lee Sun-Young, Mott Joni D, Cominetti Marcia R
Department of Gerontology, Federal University of São Carlos, São Carlos, SP, Brazil.
Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California.
J Cell Biochem. 2017 Sep;118(9):2693-2699. doi: 10.1002/jcb.25906. Epub 2017 May 15.
Breast cancer is a complex and multifactorial disease. Tumors have a heterogeneous microenvironment, which have multiple interactions with other cell types, greatly influencing the behavior of tumor cells and response to therapy. The 3D culture mimics the microenvironment better found in vivo and is more appropriated than the traditional 2D culture made from plastic to test the cellular response to drugs. To investigate the effects of [10]-gingerol on breast tumor cells, we used physiologically relevant three-dimensional (3D) cultures of malignant and non-malignant human breast cells grown in laminin-rich extracellular matrix gels (lr-ECM). Our results showed selective cytotoxicity of [10]-gingerol against the malignant T4-2 breast cancer cell line compared to non-malignant S1 cells. The compound reverted the malignant phenotype of the cancer cells, downregulating the expression of epidermal growth factor receptor (EGFR) and β1-integrin. Moreover, [10]-gingerol induced apoptosis in this cell line. These results suggest that [10]-gingerol may be an effective compound to use as adjuvant therapy in breast cancer treatment. J. Cell. Biochem. 118: 2693-2699, 2017. © 2017 Wiley Periodicals, Inc.
乳腺癌是一种复杂的多因素疾病。肿瘤具有异质性微环境，与其他细胞类型存在多种相互作用，极大地影响肿瘤细胞的行为和对治疗的反应。三维培养能更好地模拟体内发现的微环境，比由塑料制成的传统二维培养更适合用于测试细胞对药物的反应。为了研究[10]-姜辣素对乳腺肿瘤细胞的影响，我们使用了在富含层粘连蛋白的细胞外基质凝胶（lr-ECM）中生长的恶性和非恶性人乳腺细胞的生理相关三维（3D）培养物。我们的结果显示，与非恶性S1细胞相比，[10]-姜辣素对恶性T4-2乳腺癌细胞系具有选择性细胞毒性。该化合物使癌细胞的恶性表型逆转，下调表皮生长因子受体（EGFR）和β1-整合素的表达。此外，[10]-姜辣素诱导该细胞系凋亡。这些结果表明，[10]-姜辣素可能是一种有效的化合物，可用于乳腺癌治疗的辅助治疗。《细胞生物化学杂志》118: 2693 - 2699, 2017。© 2017威利期刊公司
