Department of Biology, Georgia State University, Atlanta, GA, 30303, USA.
School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Sci Rep. 2020 Nov 5;10(1):19182. doi: 10.1038/s41598-020-75707-0.
Ginger (Zingiber officinale) is one of the most widely consumed dietary supplements worldwide. Its anticancer potential has been demonstrated in various studies. However, ginger roots obtained from different geographical locations showed extensive variability in their activities, mainly due to differences in the levels of bioactive compounds. Here we evaluated the effect of these differences on the anticancer activity of ginger by performing efficacy-based fingerprinting. We characterized the fingerprint profiles of 22 ginger samples using liquid chromatography-mass spectroscopy, followed by a principal component analysis (PCA) and pearson correlation analysis. We also evaluated the anti-proliferative effects (IC) of these samples on triple-negative breast cancer cells using the MTT assays. The supervised PCA identified a subset of analytes whose abundance strongly associated with the IC values of the ginger extracts, providing a link between ginger extract composition and in vitro anticancer efficacy. This study demonstrated that variation in the ginger fingerprint profiles resulting from differences in their chemical composition could have a significant impact on efficacy and bioactivity of ginger extracts. Also, this first-of-a-kind efficacy-based fingerprinting approach proposed here can identify potent anticancer candidates from the ginger fingerprint without the need for isolating individual components from the extracts.
姜（Zingiber officinale）是世界上最广泛食用的膳食补充剂之一。它的抗癌潜力已在各种研究中得到证实。然而，来自不同地理位置的姜根在其活性方面表现出广泛的可变性，主要是由于生物活性化合物水平的差异。在这里，我们通过基于功效的指纹图谱评估了这些差异对姜抗癌活性的影响。我们使用液相色谱-质谱法对 22 个姜样本进行了指纹图谱特征描述，随后进行了主成分分析（PCA）和 Pearson 相关性分析。我们还使用 MTT 测定法评估了这些样品对三阴性乳腺癌细胞的抗增殖作用（IC）。有监督的 PCA 确定了一组分析物，其丰度与姜提取物的 IC 值强烈相关，为姜提取物成分与体外抗癌功效之间提供了联系。这项研究表明，由于化学成分的差异导致的姜指纹图谱的变化可能会对姜提取物的功效和生物活性产生重大影响。此外，这里提出的这种基于功效的指纹图谱方法可以在不需要从提取物中分离单个成分的情况下，从姜指纹图谱中识别出有效的抗癌候选物。
