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通过检测性染色体进行活检标本鉴定：原位杂交技术的应用

Biopsy specimen identification by detection of sex chromosomes: application of in situ hybridisation.

作者信息

Kibbelaar R E, Leenheers-Binnendijk C F, Spaander P J, Kluin P M

机构信息

Laboratory of Pathology, State University Leiden, The Netherlands.

出版信息

J Clin Pathol. 1992 Feb;45(2):149-50. doi: 10.1136/jcp.45.2.149.

DOI:10.1136/jcp.45.2.149
PMID:1541695
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC495661/
Abstract

AIMS

To investigate the feasibility of non-radioactive in situ hybridisation (ISH) for the identification of sex-mismatched plastic embedded bone marrow biopsy specimens.

METHODS

After a suspected accidental transposition of two glycol-methacrylate embedded bone marrow specimens, in situ hybridisation with sex chromosome specific probes was performed.

RESULTS

Quantitative analysis of the hybridisation signals established unequivocably the origin of the specimens.

CONCLUSIONS

ISH is feasible on GMA embedded bone marrow specimens, and can be used for the identification of accidentally transposed specimens provided that they are of sex-matched origin.

摘要

目的

研究非放射性原位杂交（ISH）用于鉴定性别不匹配的塑料包埋骨髓活检标本的可行性。

方法

在怀疑两个乙二醇甲基丙烯酸酯包埋的骨髓标本意外换位后，进行了性染色体特异性探针的原位杂交。

结果

杂交信号的定量分析明确确定了标本的来源。

结论

ISH在GMA包埋的骨髓标本上是可行的，并且可用于鉴定意外换位的标本，前提是它们来自性别匹配的个体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfcc/495661/cccb4b2767ce/jclinpath00416-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfcc/495661/cccb4b2767ce/jclinpath00416-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfcc/495661/cccb4b2767ce/jclinpath00416-0060-a.jpg

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Characterisation of a human Y chromosome repeated sequence and related sequences in higher primates.人类Y染色体重复序列及高等灵长类相关序列的特征分析
Chromosoma. 1982;87(5):491-502. doi: 10.1007/BF00333470.
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Isolation and characterization of a major tandem repeat family from the human X chromosome.人类X染色体上一个主要串联重复序列家族的分离与特性分析。
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Detection of chromosome aneuploidy in interphase nuclei from human primary breast tumors using chromosome-specific repetitive DNA probes.
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