Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Luojia Hill, Wuhan 430072, PR China.
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Luojia Hill, Wuhan 430072, PR China.
Carbohydr Polym. 2017 Mar 15;160:18-25. doi: 10.1016/j.carbpol.2016.12.032. Epub 2016 Dec 19.
Photocrosslinked hydrogels are being investigated for many tissue engineering applications because of the ability to form these materials in-situ in a minimally invasive manner by injection of aqueous solution under physiological conditions. In this work, carboxymethyl chitin (CMCH) synthesized homogenously was further modified with methacrylic anhydride and photocrosslinked into hydrogel with tunable degradation and mechanical properties. This new methacrylated carboxymethyl chitin (Me-CMCH) hydrogel formed in-situ photocrosslinked under UV irradiation showed much higher storage modulus than that of the thermosensitive in-situ forming physical-crosslinking CMCH hydrogel. The Me-CMCH hydrogels remained stable under physiological conditions and could be degraded by lysozyme. Cytotoxicity test indicated that the photo-induced Me-CMCH hydrogels were non-cytotoxic. The mechanical property, morphology, swelling and biodegradation behavior of the Me-CMCH hydrogels could be tuned by controlling the degree of methacrylation of Me-CMCH. These biodegradable photocrosslinkable Me-CMCH hydrogels may hold great promises for various biomedical applications.
光交联水凝胶由于能够在生理条件下通过注射水溶液以微创的方式原位形成这些材料，因此正在被广泛研究用于许多组织工程应用。在这项工作中，均匀合成的羧甲基壳聚糖（CMCH）进一步用甲基丙烯酰酐进行修饰，并通过光交联原位形成具有可调降解和机械性能的水凝胶。在紫外光照射下原位光交联形成的新型甲基丙烯酰化羧甲基壳聚糖（Me-CMCH）水凝胶的储能模量明显高于热敏感原位形成的物理交联 CMCH 水凝胶。Me-CMCH 水凝胶在生理条件下保持稳定，并且可以被溶菌酶降解。细胞毒性试验表明，光诱导的 Me-CMCH 水凝胶无细胞毒性。通过控制 Me-CMCH 的甲基丙烯酰化程度可以调节 Me-CMCH 水凝胶的机械性能、形态、溶胀和生物降解行为。这些可生物降解的光交联 Me-CMCH 水凝胶可能在各种生物医学应用中具有广阔的应用前景。
