Key Laboratory for Molecular Genetic Mechanisms and Intervention Research on High Altitude Disease of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
Key Laboratory of High Altitude Environment and Genes Related to Diseases of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, China.
Front Cell Infect Microbiol. 2021 Sep 16;11:732613. doi: 10.3389/fcimb.2021.732613. eCollection 2021.
The human oral microbiota plays a vital role in maintaining metabolic homeostasis. To explore the relationship between () and reflux esophagitis, we collected 86 saliva samples from reflux esophagitis patients (RE group) and 106 saliva samples from healthy people (C group) for a high-throughput sequencing comparison. No difference in alpha diversity was detected between the RE and the C groups, but beta diversity of the RE group was higher than the C group. Bacteroidetes was more abundant in the RE group, whereas Firmicutes was more abundant in the C group. The linear discriminant analysis effect size analysis demonstrated that the biomarkers of the RE group were , , , and , and the biomarkers of the C group were , , , and . The oral microbial network structure of the C group was more complex than that of the RE group. Second, to explore the effect of on the oral microbiota of RE patients, we performed the C-urea breath test on 45 of the 86 RE patients. We compared the oral microbiota of 33 -infected reflux esophagitis patients (REHpp group) and 12 non-Hp-infected reflux esophagitis patients (REHpn group). No difference in alpha diversity was observed between the REHpn and REHpp groups, and beta diversity of the REHpp group was significantly lower than that of the REHpn group. The biomarkers in the REHpp group were , , , , , , and ; and the biomarker in the REHpn group was . was positively correlated with in the microbial network of the REHpp group. The main finding of this study is that RE disturbs the human oral microbiota, such as increased beta diversity. infection may inhibit this disorderly trend.
人类口腔微生物群在维持代谢稳态方面起着至关重要的作用。为了探讨（）与反流性食管炎的关系，我们收集了 86 例反流性食管炎患者（RE 组）和 106 例健康人（C 组）的唾液样本进行高通量测序比较。RE 组和 C 组的 alpha 多样性无差异，但 RE 组的 beta 多样性高于 C 组。RE 组中拟杆菌门较多，而 C 组中厚壁菌门较多。线性判别分析效应量分析表明，RE 组的生物标志物为（）、（）、（）和（），C 组的生物标志物为（）、（）、（）和（）。C 组的口腔微生物网络结构比 RE 组更复杂。其次，为了探讨对 RE 患者口腔微生物群的影响，我们对 86 例 RE 患者中的 45 例进行了 C-尿素呼气试验。我们比较了 33 例幽门螺杆菌感染性反流性食管炎患者（REHpp 组）和 12 例非幽门螺杆菌感染性反流性食管炎患者（REHpn 组）的口腔微生物群。REHpn 组和 REHpp 组的 alpha 多样性无差异，REHpp 组的 beta 多样性明显低于 REHpn 组。REHpp 组的生物标志物为（）、（）、（）、（）、（）、（）和（）；REHpn 组的生物标志物为（）。在 REHpp 组的微生物网络中，与呈正相关。本研究的主要发现是 RE 扰乱了人类口腔微生物群，如增加了 beta 多样性。感染可能抑制这种紊乱趋势。
