一种小泛素样修饰蛋白连接酶AtMMS21在根发育过程中调节染色质重塑因子BRAHMA的稳定性。
A SUMO Ligase AtMMS21 Regulates the Stability of the Chromatin Remodeler BRAHMA in Root Development.
作者信息
Zhang Juanjuan, Lai Jianbin, Wang Feige, Yang Songguang, He Zhipeng, Jiang Jieming, Li Qingliang, Wu Qian, Liu Yiyang, Yu Mengyuan, Du Jinju, Xie Qi, Wu Keqiang, Yang Chengwei
机构信息
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China (J.Z., J.L., F.W., Z.H., J.J., Q.W., Y.L., M.Y., J.D., C.Y.).
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China (S.Y.).
出版信息
Plant Physiol. 2017 Mar;173(3):1574-1582. doi: 10.1104/pp.17.00014. Epub 2017 Jan 23.
Chromatin remodeling is essential for gene expression regulation in plant development and response to stresses. Brahma (BRM) is a conserved ATPase in the SWI/SNF chromatin remodeling complex and is involved in various biological processes in plant cells, but the regulation mechanism on BRM protein remains unclear. Here, we report that BRM interacts with AtMMS21, a SUMO ligase in Arabidopsis (). The interaction was confirmed in different approaches in vivo and in vitro. The mutants of and displayed a similar defect in root development. In the mutant, the protein level of BRM-GFP was significantly lower than that in wild type, but the RNA level of did not change. Biochemical evidence indicated that BRM was modified by SUMO3, and the reaction was enhanced by AtMMS21. Furthermore, overexpression of wild-type AtMMS21 but not the mutated AtMMS21 without SUMO ligase activity was able to recover the stability of BRM in Overexpression of in partially rescued the developmental defect of roots. Taken together, these results supported that AtMMS21 regulates the protein stability of BRM in root development.
染色质重塑对于植物发育和应激反应中的基因表达调控至关重要。Brahma（BRM）是SWI/SNF染色质重塑复合物中一种保守的ATP酶，参与植物细胞的各种生物学过程，但BRM蛋白的调控机制仍不清楚。在这里，我们报道BRM与拟南芥中的SUMO连接酶AtMMS21相互作用。这种相互作用在体内和体外的不同方法中得到了证实。AtMMS21和BRM的突变体在根发育中表现出类似的缺陷。在AtMMS21突变体中，BRM-GFP的蛋白水平显著低于野生型，但BRM的RNA水平没有变化。生化证据表明BRM被SUMO3修饰，并且AtMMS21增强了该反应。此外，野生型AtMMS21的过表达而非没有SUMO连接酶活性的突变型AtMMS21能够恢复AtMMS21突变体中BRM的稳定性。AtMMS21在BRM突变体中的过表达部分挽救了根的发育缺陷。综上所述，这些结果支持AtMMS21在根发育中调节BRM的蛋白稳定性。
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