Oh Hyemin, Kim Sejeong, Lee Soomin, Lee Heeyoung, Ha Jimyeong, Lee Jeeyeon, Choi Yukyung, Choi Kyoung-Hee, Yoon Yohan
Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea; Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
Department of Oral Microbiology, College of Dentistry, Wonkwang University, Iksan 54538, Korea.
Korean J Food Sci Anim Resour. 2016;36(6):779-786. doi: 10.5851/kosfa.2016.36.6.779. Epub 2016 Dec 31.
This study aimed to evaluate the prevalence of on livestock farms in Korea and determine their serotypes and genetic correlations. Twenty-five livestock farms in Korea (central: 15, south west: 7, south east: 3) were visited 2-3 times, and 2,018 samples (feces: 677, soil: 680, silage: 647, sludge: 14) were collected. Samples were enriched in LEB (Listeria enrichment broth) and Fraser broth media, and then plated on Palcam agar. The isolates were identified by PCR and 16S rRNA gene sequencing. Then, the serotypes, presence of virulence genes (, , , , and ), and antibiotic resistance were determined. Genetic correlations among the isolates were evaluated by analyzing the restriction digest pattern with I. Of the 2,018 samples, only 3 (0.15%) soil samples (FI-1-FI-3) from 1 farm in the south east region were positive for . Based on biochemical tests and multiplex PCR, the serotype of the isolates were 4ab (FI-1 and FI-3) and 3a (FI-2), which are not common in foodborne . The 3a serotype isolate was positive for all tested virulence genes, whereas the 4ab serotype isolates were only positive for , , and . The isolates were resistant to all 12 tested antibiotics, especially FI-3. The genetic correlations among the isolates were 100% for those of the same serotype and 26.3% for those of different serotypes. These results indicate that the prevalence of on livestock farms in Korea is low; however, the isolates are pathogenic and antibiotic resistant.
本研究旨在评估韩国畜牧场中[具体细菌名称未给出]的流行情况，并确定其血清型和遗传相关性。对韩国的25个畜牧场（中部：15个，西南部：7个，东南部：3个）进行了2至3次走访，共采集了2018份样本（粪便：677份，土壤：680份，青贮饲料：647份，污泥：14份）。样本在LEB（李斯特菌增菌肉汤）和弗雷泽肉汤培养基中增菌，然后接种于Palcam琼脂平板上。通过PCR和16S rRNA基因测序对分离株进行鉴定。随后，确定血清型、毒力基因（[具体毒力基因未给出]）的存在情况以及抗生素耐药性。通过用[具体酶未给出]分析限制性消化图谱来评估分离株之间的遗传相关性。在2018份样本中，仅来自东南部地区1个农场的3份（0.15%）土壤样本（FI - 1 - FI - 3）对[具体细菌名称未给出]呈阳性。基于生化试验和多重PCR，分离株的血清型为4ab（FI - 1和FI - 3）和3a（FI - 2），这在食源性[具体细菌名称未给出]中并不常见。3a血清型分离株对所有测试的毒力基因均呈阳性，而4ab血清型分离株仅对[部分毒力基因未给出]呈阳性。这些分离株对所有12种测试抗生素均耐药，尤其是FI - 3。相同血清型的分离株之间遗传相关性为100%，不同血清型的分离株之间遗传相关性为26.3%。这些结果表明，韩国畜牧场中[具体细菌名称未给出]的流行率较低；然而，分离株具有致病性且对抗生素耐药。
