Klonowska Agnieszka, López-López Aline, Moulin Lionel, Ardley Julie, Gollagher Margaret, Marinova Dora, Tian Rui, Huntemann Marcel, Reddy T B K, Varghese Neha, Woyke Tanja, Markowitz Victor, Ivanova Natalia, Seshadri Rekha, Baeshen Mohamed N, Baeshen Nabih A, Kyrpides Nikos, Reeve Wayne
IRD, Cirad, Univ. Montpellier, Interactions Plantes Microorganismes Environnement (IPME), 34394 Montpellier, France ; IRD, UMR LSTM-Laboratoire des Symbioses Tropicales et Méditerranéennes, 34398 Montpellier cedex 5, France.
IRD, UMR LSTM-Laboratoire des Symbioses Tropicales et Méditerranéennes, 34398 Montpellier cedex 5, France.
Stand Genomic Sci. 2017 Jan 17;12:7. doi: 10.1186/s40793-016-0212-4. eCollection 2017.
STM6155 (INSCD = ATYY01000000) is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as an effective nitrogen fixing microsymbiont of the legume L.. STM6155 was isolated in 2009 from a nodule of the trap host grown in nickel-rich soil collected near Mont Dore, New Caledonia. STM6155 was selected as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) genome sequencing project. Here we describe the symbiotic properties of STM6155, together with its genome sequence information and annotation. The 6,927,906 bp high-quality draft genome is arranged into 147 scaffolds of 152 contigs containing 6855 protein-coding genes and 71 RNA-only encoding genes. Strain STM6155 forms an ANI clique (ID 2435) with the sequenced strain STM3625, and the nodulation genes are highly conserved in these strains and the type strain of CCGE501. Within the STM6155 genome, we have identified a chromate efflux gene cluster of six genes arranged into two putative operons and we postulate that this cluster is important for the survival of STM6155 in ultramafic soils containing high concentrations of chromate.
STM6155（INSCD = ATYY01000000）是一种需氧、能运动、革兰氏阴性、不形成芽孢的杆菌，它既可以作为土壤腐生菌存在，也可以作为豆科植物L.的高效固氮微共生体。STM6155于2009年从新喀里多尼亚蒙特多雷附近采集的富含镍土壤中生长的诱捕宿主的根瘤中分离出来。STM6155被选为美国能源部联合基因组研究所2010年细菌和古细菌基因组百科全书——根瘤菌（GEBA - RNB）基因组测序项目的一部分。在此，我们描述了STM6155的共生特性，以及它的基因组序列信息和注释。这个6,927,906 bp的高质量草图基因组被排列成147个支架，由152个重叠群组成，包含6855个蛋白质编码基因和71个仅编码RNA的基因。菌株STM6155与已测序的菌株STM3625形成一个ANI簇（ID 2435），并且这些菌株和CCGE501模式菌株中的结瘤基因高度保守。在STM6155基因组中，我们鉴定出一个由六个基因组成的铬酸盐外排基因簇，这些基因排列成两个假定的操纵子，我们推测这个基因簇对于STM6155在含有高浓度铬酸盐的超基性土壤中的生存至关重要。
