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脂多糖与多聚精氨酸通过 JNK 信号通路协同促进 NCI-H292 细胞释放白细胞介素 6 和白细胞介素 8。

LPS Cooperates with Poly-L-Arginine to Promote IL-6 and IL-8 Release via the JNK Signaling Pathway in NCI-H292 Cells.

机构信息

Department of Geriatric Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.

Department of Biochemistry and Molecular Biology, Anhui Medical University, Number 81, Meishan Road, Hefei, Anhui 230022, China.

出版信息

J Immunol Res. 2016;2016:3421060. doi: 10.1155/2016/3421060. Epub 2016 Dec 26.

DOI:10.1155/2016/3421060
PMID:28116315
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5220512/
Abstract

. Herein, we aimed to study the mechanism whereby poly-L-arginine (PLA) and lipopolysaccharide (LPS) can synergistically induce the release of interleukin-6 (IL-6) and IL-8 in NCI-H292 cells. . NCI-H292 cells were divided into control, PLA, LPS, and PLA+LPS groups. At various time points, the phosphorylation of JNK in each group was measured by western blotting. Additionally, the productions of IL-6 and IL-8 were assessed using an enzyme-linked immunosorbent assay (ELISA). The effects of SP600125, an inhibitor of the JNK pathway, on the increase of p-JNK, IL-6, and IL-8 were also studied. . Our results showed that either PLA or LPS treatment alone can significantly increase the phosphorylation level of JNK in NCI-H292 cells. Of interest was the combined use of PLA and LPS that has a synergistic effect on the phosphorylation of JNK, as well as synergistically inducing the release of IL-6 and IL-8 in NCI-H292 cells. Furthermore, SP600125 significantly inhibited the activation of JNK signal, as well as reducing the productions of IL-6 and IL-8 in response to PLA+LPS stimulation. . The JNK signaling pathway contributes to the release of IL-6 and IL-8, which is stimulated by the synergistic actions of PLA+LPS in NCI-H292 cells.

摘要

. 本研究旨在探讨多聚左旋精氨酸（PLA）和脂多糖（LPS）协同诱导 NCI-H292 细胞释放白细胞介素-6（IL-6）和白细胞介素-8（IL-8）的机制。. 将 NCI-H292 细胞分为对照组、PLA 组、LPS 组和 PLA+LPS 组。在不同时间点，通过 Western blot 法测定各组 JNK 的磷酸化水平。此外，采用酶联免疫吸附试验（ELISA）检测 IL-6 和 IL-8 的产生量。还研究了 JNK 通路抑制剂 SP600125 对 p-JNK、IL-6 和 IL-8 增加的影响。. 结果显示，单独使用 PLA 或 LPS 均可显著增加 NCI-H292 细胞 JNK 的磷酸化水平。有趣的是，PLA 和 LPS 联合使用对 JNK 的磷酸化具有协同作用，并协同诱导 NCI-H292 细胞释放 IL-6 和 IL-8。此外，SP600125 显著抑制 JNK 信号的激活，并减少 PLA+LPS 刺激引起的 IL-6 和 IL-8 的产生。. JNK 信号通路参与 PLA+LPS 刺激 NCI-H292 细胞释放 IL-6 和 IL-8，其作用具有协同性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30bb/5220512/3a701d8c1a3c/JIR2016-3421060.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30bb/5220512/e27741d3b2ec/JIR2016-3421060.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30bb/5220512/09e1708f89be/JIR2016-3421060.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30bb/5220512/3a701d8c1a3c/JIR2016-3421060.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30bb/5220512/e27741d3b2ec/JIR2016-3421060.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30bb/5220512/09e1708f89be/JIR2016-3421060.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/30bb/5220512/3a701d8c1a3c/JIR2016-3421060.003.jpg

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