College of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea; Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
Department of Science in Korean Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea; Department of Biological Sciences in Korean Medicine, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
J Immunol Res. 2016;2016:7521601. doi: 10.1155/2016/7521601. Epub 2016 Dec 27.
. There is limited information of the anti-inflammatory effects of Rg3 on inflamed lung cells and tissues. Therefore, we confirmed the anti-inflammatory mechanism of ginsenoside Rg3 in inflamed human airway epithelial cells (A549) and tissues whether Rg3 regulates nuclear factor kappa B (NF-B) activity. . To induce the inflammation, IL-1 (10 ng/ml) was treated to A549 cells for 4 h. The effects of Rg3 on NF-B activity and COX-2 expression were evaluated by western blotting analysis in both IL-1-induced inflamed A549 cell and human asthmatic airway epithelial tissues. Using multiplex cytokines assay, the secretion levels of NF-B-mediated cytokines/chemokines were measured. . Rg3 showed the significant inhibition of NF-B activity thereby reduced COX-2 expression was determined in both IL-1-induced inflamed A549 cell and human asthmatic airway epithelial tissues. In addition, among NF-B-mediated cytokines, the secretion levels of IL-4, TNF-, and eotaxin were significantly decreased by Rg3 in asthma tissues. Even though there was no significant difference, IL-6, IL-9, and IL-13 secretion showed a lower tendency compared to saline-treated human asthmatic airway epithelial tissues. . The results from this study demonstrate the potential of Rg3 as an anti-inflammatory agent through regulating NF-B activity and reducing the secretion of NF-B-mediated cytokines/chemokines.
. 关于 Rg3 对炎症肺细胞和组织的抗炎作用的信息有限。因此，我们确认了人参皂苷 Rg3 在炎症人气道上皮细胞（A549）和组织中的抗炎机制，即 Rg3 是否调节核因子 kappa B（NF-B）活性。. 为了诱导炎症，用 IL-1（10ng/ml）处理 A549 细胞 4 小时。通过 Western blot 分析评估 Rg3 对 IL-1 诱导的炎症 A549 细胞和人哮喘气道上皮组织中 NF-B 活性和 COX-2 表达的影响。通过多重细胞因子测定法测量 NF-B 介导的细胞因子/趋化因子的分泌水平。. Rg3 显示出对 NF-B 活性的显著抑制作用，从而降低了 COX-2 的表达，这在 IL-1 诱导的炎症 A549 细胞和人哮喘气道上皮组织中均得到了证实。此外，在 NF-B 介导的细胞因子中，Rg3 显著降低了哮喘组织中 IL-4、TNF-和 eotaxin 的分泌水平。尽管没有显著差异，但与用生理盐水处理的人哮喘气道上皮组织相比，IL-6、IL-9 和 IL-13 的分泌表现出较低的趋势。. 这项研究的结果表明，Rg3 作为一种抗炎剂，通过调节 NF-B 活性和减少 NF-B 介导的细胞因子/趋化因子的分泌，具有潜在的应用前景。
