a College of Korean Medicine , Kyung Hee University , Seoul , Republic of Korea.
b Department of Science in Korean Medicine , Graduate School, Kyung Hee University , Seoul , Republic of Korea.
Immunopharmacol Immunotoxicol. 2018 Feb;40(1):83-90. doi: 10.1080/08923973.2017.1414836. Epub 2018 Jan 4.
Allergic asthma is the most common type in asthma, which is defined as a chronic inflammatory disease of the lung. In this study, we investigated whether embelin (Emb), the major component of Ardisia japonica BL. (AJB), exhibits anti-inflammatory effects on allergic asthma via inhibition of NF-κB activity using A549 cells and asthmatic airway epithelial tissues.
Inflammation was induced in A549 cells, a human airway epithelial cell line, by IL-1β (10 ng/ml) treatment for 4 h. The effects of Emb on NF-κB activity and COX-2 protein expression in inflamed airway epithelial cells and human asthmatic airway epithelial tissues were analyzed via western blot. The secretion levels of NF-κB-mediated cytokines/chemokines, including IL-4, 6, 9, 13, TNF-α and eotaxin, were measured by a multiplex assay.
Emb significantly blocked NF-κB activity in IL-1β-treated A549 cells and human asthmatic airway epithelial tissues. COX-2 expression was also reduced in both IL-1β-treated A549 cells and asthmatic tissues Emb application. Emb significantly reduced the secretion of IL-4, IL-6 and eotaxin in human asthmatic airway epithelial tissues by inhibiting activity of NF-κB.
The results of this study suggest that Emb may be used as an anti-inflammatory agent via inhibition of NF-κB and related cytokines.
过敏性哮喘是哮喘中最常见的类型，被定义为肺部的慢性炎症性疾病。在这项研究中，我们通过使用 A549 细胞和哮喘气道上皮组织，研究了 Ardisia japonica BL.（AJB）的主要成分杨梅素（Emb）是否通过抑制 NF-κB 活性对过敏性哮喘表现出抗炎作用。
通过用 IL-1β（10ng/ml）处理 4 小时，在人气道上皮细胞系 A549 细胞中诱导炎症。通过 Western blot 分析 Emb 对炎性气道上皮细胞和人哮喘气道上皮组织中 NF-κB 活性和 COX-2 蛋白表达的影响。通过多重分析测定 NF-κB 介导的细胞因子/趋化因子（包括 IL-4、6、9、13、TNF-α 和 eotaxin）的分泌水平。
Emb 显著抑制了 IL-1β 处理的 A549 细胞和人哮喘气道上皮组织中的 NF-κB 活性。在 IL-1β 处理的 A549 细胞和哮喘组织中，COX-2 的表达也降低。Emb 通过抑制 NF-κB 的活性，显著减少了人哮喘气道上皮组织中 IL-4、IL-6 和 eotaxin 的分泌。
这项研究的结果表明，Emb 可通过抑制 NF-κB 和相关细胞因子用作抗炎剂。
