Rios Francisco J, Touyz Rhian M, Montezano Augusto C
Institute of Cardiovascular and Medical Sciences, British Heart Foundation Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, UK.
Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.
Methods Mol Biol. 2017;1527:311-320. doi: 10.1007/978-1-4939-6625-7_24.
Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis development. In the heart, vascular tissues and perivascular adipose tissues, there is increasing evidence that macrophages play a role in endothelial dysfunction, vascular inflammation and remodeling. In this chapter we describe protocols to isolate human monocytes from peripheral blood mononuclear cell and how to differentiate them into macrophages by using growth factors (granulocyte-macrophage colony-stimulating factor-GM-CSF or colony-stimulating factor-M-CSF) or by different cell culture medium concentration. We also describe how to indentify different macrophage activation phenotypes.
巨噬细胞亚型在血管病变中表现出保护或致病活性。它们识别并吞噬修饰的脂质，积聚在动脉内膜，促进动脉粥样硬化的发展。在心脏、血管组织和血管周围脂肪组织中，越来越多的证据表明巨噬细胞在内皮功能障碍、血管炎症和重塑中发挥作用。在本章中，我们描述了从外周血单核细胞中分离人单核细胞的方法，以及如何通过使用生长因子（粒细胞-巨噬细胞集落刺激因子-GM-CSF或集落刺激因子-M-CSF）或通过不同的细胞培养基浓度将它们分化为巨噬细胞。我们还描述了如何鉴定不同的巨噬细胞激活表型。
