Micro/Nanofabrication facility, Department of Mechanical and Industrial Engineering, Ryerson University , 350 Victoria street, Toronto, Ontario M5B 2K3, Canada.
Affiliate Scientist, Keenan Research Center, St. Michael's Hospital , 209 Victoria Street, Toronto, Ontario M5B 1T8, Canada.
ACS Appl Mater Interfaces. 2017 Feb 15;9(6):5077-5091. doi: 10.1021/acsami.6b13576. Epub 2017 Feb 3.
We introduce a "non-noble metal" based SERS active nanobiosensor using a self-assembled 3D hybrid nickel nanonetwork. A tunable biomolecule detector fabricated by a bottom-up approach was functionalized using a multiphoton ionization energy mechanism to create a self-assembled 3D hybrid nickel nanonetwork. The nanonetwork was tested for SERS detection of crystal violet (CV) and glutathione (GSH) at two excitation wavelengths, 532 and 785 nm. The results reveal indiscernible peaks with a limit of detection (LOD) of 1 picomolar (pM) concentration. An enhancement factor (EF) of 9.3 × 10 was achieved for the chemical molecule CV and 1.8 × 10 for the biomolecule GSH, which are the highest reported values so far. The two results, one being the CV molecule proved that nickel nanonetwork is indeed SERS active and the second being the GSH biomolecule detection at both 532 and 785 nm, confirm that the nanonetwork is a biosensor which has potential for both in vivo and in vitro sensing. In addition, the selectivity and versatility of this biosensor is examined with biomolecules such as l-Cysteine, l-Methionine, and sensing GSH in cell culture medium which mimics the complex biological environment. The functionalized self-assembled 3D hybrid nickel nanonetwork exhibits electromagnetic and charge transfer based SERS activation mechanisms.
我们介绍了一种基于非贵金属的 SERS 活性纳米生物传感器，该传感器使用自组装的 3D 混合镍纳米网。通过自下而上的方法制造的可调谐生物分子探测器通过多光子电离能机制进行功能化，以创建自组装的 3D 混合镍纳米网。该纳米网用于在 532 和 785nm 两个激发波长下对结晶紫 (CV) 和谷胱甘肽 (GSH) 的 SERS 检测进行了测试。结果表明，在 1 皮摩尔 (pM) 浓度下，检测到的峰无法区分。对于化学分子 CV，获得了 9.3×10 的增强因子 (EF)，对于生物分子 GSH，获得了 1.8×10 的增强因子 (EF)，这是迄今为止报道的最高值。这两个结果，一个是 CV 分子，证明镍纳米网确实具有 SERS 活性，另一个是在 532nm 和 785nm 下都能检测到 GSH 生物分子，这证实了纳米网是一种生物传感器，具有在体内和体外传感的潜力。此外，还通过 l-半胱氨酸、l-蛋氨酸等生物分子以及模拟复杂生物环境的细胞培养基中 GSH 的传感来检查这种生物传感器的选择性和多功能性。功能化的自组装 3D 混合镍纳米网表现出基于电磁和电荷转移的 SERS 激活机制。
