Yu Shihua, Liu Zhigang, Zhang Jianpo, Li Hongwei, Xu Na, Yuan Xin-Xin, Wu Yuqing
State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University 2699 Qianjin Avenue Changchun 130012 China
College of Chemical & Pharmaceutical Engineering, Jilin Institute of Chemical Technology Jilin 132022 China.
RSC Adv. 2018 Feb 1;8(10):5536-5541. doi: 10.1039/c7ra12927k. eCollection 2018 Jan 29.
To date, great achievements with GC-MS, HPLC-MS, and fluorescence biosensing techniques have been made to detect illegal additives of salbutamol (SAL) in swine meat. However, these methods are not suitable for rapid on-site screening due to either costly instruments or rather complicated and/or time consuming sample pretreatments. Herein, a simple, rapid and ultrasensitive approach based on an azo-coupling reaction and surface-enhanced resonance Raman scattering (SERRS) is presented. By combining with a magnetic SERS substrate, an indirect detection for SAL, with a LOD of 1.0 × 10 M (2.39 pg mL), was realized. Moreover, a colorimetric method for naked eye detection was successfully carried out for rapid screening of SAL in concentrations higher than 2.09 × 10 M (5 μg mL). In addition, the proposed method was successfully applied for the rapid determination of SAL in real swine meat. The entire process, including pretreatment, coupling reaction and SERRS detection, was performed within 7 min. Moreover, the SERRS fingerprint band being specific to corresponding functional group guarantees the selectivity for the target molecule. Therefore, the proposed strategy in the present study offers a new way to identify trace amounts of analytes, such as SAL as well as other illegal additives in health-related products and food.
迄今为止，在检测猪肉中非法添加的沙丁胺醇（SAL）方面，气相色谱 - 质谱联用（GC-MS）、高效液相色谱 - 质谱联用（HPLC-MS）和荧光生物传感技术已经取得了巨大成就。然而，由于仪器成本高昂或样品预处理相当复杂和/或耗时，这些方法不适用于快速现场筛查。在此，提出了一种基于偶氮偶联反应和表面增强共振拉曼散射（SERRS）的简单、快速且超灵敏的方法。通过与磁性表面增强拉曼散射（SERS）基底相结合，实现了对SAL的间接检测，检测限为1.0×10⁻¹¹ M（2.39 pg/mL）。此外，成功开发了一种用于肉眼检测的比色法，用于快速筛查浓度高于2.09×10⁻⁸ M（5 μg/mL）的SAL。此外，所提出的方法成功应用于实际猪肉中SAL的快速测定。整个过程，包括预处理、偶联反应和SERRS检测，在7分钟内完成。此外，特定于相应官能团的SERRS指纹带保证了对目标分子的选择性。因此，本研究中提出的策略为识别痕量分析物提供了一种新方法，例如SAL以及健康相关产品和食品中的其他非法添加剂。
