Department of Pharmacology, Baroda College of Pharmacy, Vadodara, Gujarat, India.
Department of Pharmacology, Baroda College of Pharmacy, Vadodara, Gujarat, India.
J Ethnopharmacol. 2017 Mar 6;199:1-8. doi: 10.1016/j.jep.2017.01.031. Epub 2017 Jan 21.
Root of Hemidesmus indicus (L). R. Br., an herbal drug of India is traditionally used to prevent or treat female hormonal disturbance related disorders and assuaging symptoms of menopausal conditions.
Lack of scientific data for potential use in bone loss, the present study was undertaken to investigate the effects of ethanol extract of Hemidesmus indicus root for osteoporosis in ovariectomised rats.
Thirty wistar female rats were randomly divided into five groups. One group was sham operated and other four groups were undergone dorsal ovariectomy (OVX). After four weeks of post-operative recovery, three OVX groups, viz. Group1, Group2 and Group3 were treated for ninety days with standard raloxifene (RLX) 1mg/kg/day, Hemidesmus indicus root extract (HIRE) 100mg/kg/day and HIRE 200mg/kg/day respectively. Sham and OVX control groups were fed with equal 1% vehicle volume. Serum and urine biochemistry (alkaline phosphatase, tartrate resistant acid phosphatase, triglyceride, total cholesterol, calcium, phosphorous and hydroxyproline), weight of body, uterus and vagina, femur parameters, three point bending of tibia and fourth lumbar vertebra compression were examined. Furthermore, bone architecture and uterine hypertrophy were examined by histopathological studies.
Application of HIRE has increased bone strength and restored bone turnover markers such as alkaline phosphatase, tartrate resistant acid phosphatase and hydroxyproline moreover, improved femur parameters indicated mineralized bones, similar to the results observed with raloxifene treatment. Histopathology studies showed fibrocartilaginous proliferation of trabecular bone and absence of uterine hypertrophy.
The results strongly suggest that HIRE prevents bone loss in OVX induced osteoporosis without estrogen like side effects. It might be a potential remedy like raloxifene for postmenopausal or estrogen deficiency caused osteoporosis.
印度草药药源印度土槿皮（L.）R. Br. 的根，传统上用于预防或治疗与女性激素失调相关的疾病，并缓解更年期症状。
由于缺乏潜在用于骨质疏松症的科学数据，本研究旨在探讨印度土槿皮根乙醇提取物对去卵巢大鼠骨质疏松症的作用。
30 只 Wistar 雌性大鼠随机分为五组。一组为假手术组，其余四组行背侧卵巢切除术（OVX）。术后 4 周恢复后，三组 OVX 组（第 1 组、第 2 组和第 3 组）分别用标准雷洛昔芬（RLX）1mg/kg/天、印度土槿皮根提取物（HIRE）100mg/kg/天和 HIRE 200mg/kg/天治疗 90 天。假手术和 OVX 对照组用等量的 1%载体体积喂养。检查血清和尿液生化（碱性磷酸酶、耐酒石酸酸性磷酸酶、甘油三酯、总胆固醇、钙、磷和羟脯氨酸）、体重、子宫和阴道、股骨参数、三点弯曲胫骨和第四腰椎压缩。此外，通过组织病理学研究检查骨结构和子宫肥大。
应用 HIRE 增加了骨强度并恢复了骨转换标志物，如碱性磷酸酶、耐酒石酸酸性磷酸酶和羟脯氨酸，此外，改善的股骨参数表明矿化骨骼，类似于雷洛昔芬治疗的结果。组织病理学研究显示，小梁骨纤维软骨增殖和子宫肥大缺失。
结果强烈表明，HIRE 可预防去卵巢诱导的骨质疏松症中的骨丢失，而无雌激素样副作用。它可能是一种潜在的补救措施，类似于雷洛昔芬，用于绝经后或雌激素缺乏引起的骨质疏松症。
