微小RNA-145通过靶向N-钙黏蛋白抑制肺腺癌细胞的侵袭和迁移。
MiRNA-145 suppresses lung adenocarcinoma cell invasion and migration by targeting N-cadherin.
作者信息
Mo Dongping, Yang Daheng, Xiao Xuelian, Sun Ruihong, Huang Lei, Xu Jian
机构信息
Department of Laboratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People's Republic of China.
National Key Clinical Department of Laboratory Medicine, Nanjing, 210029, China.
出版信息
Biotechnol Lett. 2017 May;39(5):701-710. doi: 10.1007/s10529-017-2290-9. Epub 2017 Jan 24.
OBJECTIVE
To investigate the roles of miR-145 in lung adenocarcinoma (LAC) and to clarify the regulation of N-cadherin by miR-145.
RESULTS
In 57 paired clinical LAC tissues, diminished miR-145 was significantly correlated with the lymph node metastasis and was negatively correlated with N-cadherin mRNA level expression. Wound healing and transwell assays revealed a reduced capability of tumor metastasis induced by miR-145 in LAC. miR-145 negatively regulated the invasion of cell lines through targeting N-cadherin by directly binding to its 3'-untranslated region. Silencing of N-cadherin inhibited invasion and migration of LAC cell lines similar to miR-145 overexpression.
CONCLUSIONS
MiR-145 could inhibit invasion and migration of lung adenocarcinoma cell lines by directly targeting N-cadherin.
目的
研究miR-145在肺腺癌（LAC）中的作用，并阐明miR-145对N-钙黏蛋白的调控作用。
结果
在57对临床LAC组织中，miR-145表达降低与淋巴结转移显著相关，且与N-钙黏蛋白mRNA水平表达呈负相关。伤口愈合实验和Transwell实验显示，miR-145可降低LAC的肿瘤转移能力。miR-145通过直接结合N-钙黏蛋白的3'-非翻译区靶向调控其表达，进而负向调节细胞系的侵袭能力。沉默N-钙黏蛋白可抑制LAC细胞系的侵袭和迁移，其作用类似于miR-145过表达。
结论
miR-145可通过直接靶向N-钙黏蛋白抑制肺腺癌细胞系的侵袭和迁移。
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