Laboratory of Molecular Nutrition, National Engineering Laboratory for Rice and Byproducts, College of Food Science and Engineering, Central South University of Forestry and Technology , Changsha, Hunan 410004, China.
Department of Gastroenterology, Xiangya Hospital, Central South University , Changsha, Hunan 410008, China.
J Agric Food Chem. 2017 May 10;65(18):3647-3658. doi: 10.1021/acs.jafc.6b05465. Epub 2017 May 1.
Octacosanol has multiple biological functions. In this study, the anti-inflammatory effect and molecular mechanism of octacosanol were evaluated by using dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice and lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells. The colitis mouse model was induced by 3.0% DSS in 8-week ICR mice and octacosanol orally administered with 100 mg/kg/day. The results showed that octacosanol significantly improved the health status of mice and reduced DSS-induced pathological damage in the colonic tissues. Octacosanol obviously inhibited the mRNA and protein expression levels of pro-inflammatory factors of colonic tissues. In vitro, octacosanol administration significantly reduced the expression of mRNA or protein of pro-inflammatory cytokines and the phosphorylation of c-Jun N-terminal kinase and p38, and it also partly prevented LPS-induced translocations of NF-κB and AP-1. Octacosanol has anti-inflammatory effect, and its molecular mechanism may be involved in downregulating the expression of inflammatory factors and blocking of MAPK/NF-κB/AP-1 signaling pathway.
二十八烷醇具有多种生物学功能。本研究采用葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎模型和脂多糖（LPS）刺激的小鼠巨噬细胞 RAW264.7 细胞，评估了二十八烷醇的抗炎作用及其分子机制。在 8 周龄 ICR 小鼠中用 3.0% DSS 诱导结肠炎模型，并以 100mg/kg/天的剂量口服二十八烷醇。结果表明，二十八烷醇显著改善了小鼠的健康状况，并减轻了 DSS 诱导的结肠组织病理损伤。二十八烷醇明显抑制了结肠组织中促炎因子的 mRNA 和蛋白表达水平。在体外，二十八烷醇给药显著降低了促炎细胞因子的 mRNA 或蛋白表达以及 c-Jun N-末端激酶和 p38 的磷酸化，并且还部分阻止了 LPS 诱导的 NF-κB 和 AP-1 的易位。二十八烷醇具有抗炎作用，其分子机制可能涉及下调炎症因子的表达和阻断 MAPK/NF-κB/AP-1 信号通路。
