Sakaki T, Tsunoda S, Kyoi K, Utsumi S, Ascher P W, Auer L M
Department of Neurosurgery, Nara Medical University, Japan.
No Shinkei Geka. 1989 Jul;17(7):641-6.
The effect of the laser energy to the cerebral vascular reactivity and the blood brain barrier. A Nd:YAG laser with 20 watt impacts of 0.5, 1.0, 2.5 and 5.0 seconds duration time were irradiated through the cranial window made at the parietal regions of anesthetized adult cats. The disruption of the blood brain barrier was examined by checking the degree of the extravasation of Evans blue dye administrated in the vein. The cortical vessel reactivity was observed through the cranial window and evaluated using an intravital microscope and a videoangiometer. The extravasation of Evans blue dye was seen uniformly extending from the histologically changed area into the surrounding tissue in all experiments. The extent of the extravasation of dye was 1 to 1.5 mm larger than the extent of the histologically changed area produced by laser irradiation. Pial arteries in the area with histological changes dilated markedly and some of them lost their blood stream. Pial arteries in the area of Evans blue extravasation, but outside it histological changes also dilated markedly. Furthermore, pial arteries within a distance of 200 to 400 microns from the edge of the Evans blue extravasation area also dilated moderately. A statistical estimation showed that the degree of dilatation of arteries in the area outside the histological change improved significantly in the time course of five minutes.
激光能量对脑血管反应性和血脑屏障的影响。使用一台功率为20瓦的Nd:YAG激光，对麻醉的成年猫顶叶区域制作的颅骨窗进行持续时间为0.5、1.0、2.5和5.0秒的冲击照射。通过检查静脉注射伊文思蓝染料的外渗程度来检测血脑屏障的破坏情况。通过颅骨窗观察皮质血管反应性，并使用活体显微镜和视频血管计进行评估。在所有实验中，均可见伊文思蓝染料从组织学改变区域均匀地延伸至周围组织。染料外渗范围比激光照射产生的组织学改变区域大1至1.5毫米。组织学改变区域的软脑膜动脉明显扩张，其中一些失去了血流。伊文思蓝外渗区域但在其外无组织学改变区域的软脑膜动脉也明显扩张。此外，距伊文思蓝外渗区域边缘200至400微米范围内的软脑膜动脉也有中度扩张。统计估计显示，在5分钟的时间进程中，组织学改变区域外动脉的扩张程度有显著改善。
