分级 NiCo₂O₄ 空心球作为过氧化物酶模拟物用于比色检测 H₂O₂ 和葡萄糖。
Hierarchical NiCo₂O₄ Hollow Sphere as a Peroxidase Mimetic for Colorimetric Detection of H₂O₂ and Glucose.
机构信息
State Key Laboratory of Marine Resource Utilization in South China Sea, Key Laboratory of Tropical Biological Resources of Ministry of Education Hainan University, College of Material and Chemical Engineering, Haikou 570228, China.
Laboratory Cultivation Base of Natural Gas Conversion, School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China.
出版信息
Sensors (Basel). 2017 Jan 23;17(1):217. doi: 10.3390/s17010217.
In this work, the hierarchical NiCo₂O₄ hollow sphere synthesized via a "coordinating etching and precipitating" process was demonstrated to exhibit intrinsic peroxidase-like activity. The peroxidase-like activity of NiCo₂O₄, NiO, and Co₃O₄ hollow spheres were comparatively studied by the catalytic oxidation reaction of 3,3,5,5-tetramethylbenzidine (TMB) in presence of H₂O₂, and a superior peroxidase-like activity of NiCo₂O₄ was confirmed by stronger absorbance at 652 nm. Furthermore, the proposed sensing platform showed commendable response to H₂O₂ with a linear range from 10 μM to 400 μM, and a detection limit of 0.21 μM. Cooperated with GOx, the developed novel colorimetric and visual glucose-sensing platform exhibited high selectivity, favorable reproducibility, satisfactory applicability, wide linear range (from 0.1 mM to 4.5 mM), and a low detection limit of 5.31 μM. In addition, the concentration-dependent color change would offer a better and handier way for detection of H₂O₂ and glucose by naked eye.
在这项工作中，通过“配位刻蚀和沉淀”工艺合成的分级 NiCo₂O₄ 空心球被证明具有内在的过氧化物酶样活性。通过在 H₂O₂存在下催化 3,3,5,5-四甲基联苯胺（TMB）的氧化反应，比较研究了 NiCo₂O₄、NiO 和 Co₃O₄空心球的过氧化物酶样活性，并且 NiCo₂O₄ 具有更强的 652nm 处吸光度，证实了其具有优越的过氧化物酶样活性。此外，所提出的传感平台对 H₂O₂表现出良好的响应，线性范围为 10 μM 至 400 μM，检测限为 0.21 μM。与 GOx 配合使用，开发的新型比色和可视化葡萄糖传感平台表现出高选择性、良好的重现性、令人满意的适用性、宽线性范围（从 0.1 mM 到 4.5 mM）和 5.31 μM 的低检测限。此外，浓度依赖性的颜色变化将为通过肉眼检测 H₂O₂和葡萄糖提供更好、更方便的方法。
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