Department of Restorative Dentistry, Faculty of Dental Surgery, University of Malta, Msida, Malta.
Department of Stomatology, Faculty of Dentistry, University of Granada, Campus Cartuja s/n, Colegio Máximo 18071, Granada, Spain.
Sci Rep. 2017 Jan 27;7:41359. doi: 10.1038/srep41359.
Dental materials based on Portland cement, which is used in the construction industry have gained popularity for clinical use due to their hydraulic properties, the interaction with tooth tissue and their antimicrobial properties. The antimicrobial properties are optimal in vitro. However in clinical use contact with blood may affect the antimicrobial properties. This study aims to assess whether antimicrobial properties of the Portland cement-based dental cements such as mineral trioxide aggregate (MTA) are also affected by contact with blood present in clinical situations. ProRoot MTA, a Portland cement-based dental cement was characterized following contact with water, or heparinized blood after 1 day and 7 days aging. The antimicrobial activity under the mentioned conditions was assessed using 3 antimicrobial tests: agar diffusion test, direct contact test and intratubular infection test. MTA in contact with blood was severely discoloured, exhibited an additional phosphorus peak in elemental analysis, no calcium hydroxide peaks and no areas of bacterial inhibition growth in the agar diffusion test were demonstrated. ProRoot MTA showed limited antimicrobial activity, in both the direct contact test and intratubular infection test. When aged in water ProRoot MTA showed higher antimicrobial activity than when aged in blood. Antimicrobial activity reduced significantly after 7 days. Further assessment is required to investigate behaviour in clinical situations.
基于波特兰水泥的牙科材料在建筑行业中得到了广泛应用，由于其水硬性、与牙组织的相互作用和抗菌性能，在临床应用中也越来越受欢迎。其抗菌性能在体外是最佳的。然而，在临床应用中，与血液接触可能会影响其抗菌性能。本研究旨在评估与临床情况下存在的血液接触是否也会影响基于波特兰水泥的牙科水门汀（如矿物三氧化物聚合体（MTA））的抗菌性能。ProRoot MTA 是一种基于波特兰水泥的牙科水门汀，在与水接触或在 1 天和 7 天后与肝素化血液接触后，对其特性进行了描述。在上述条件下，使用 3 种抗菌试验：琼脂扩散试验、直接接触试验和管内感染试验，评估了其抗菌活性。接触血液的 MTA 严重变色，元素分析显示出现额外的磷峰，琼脂扩散试验中没有氢氧化钙峰，也没有显示出细菌抑制生长的区域。ProRoot MTA 在直接接触试验和管内感染试验中显示出有限的抗菌活性。在水中老化时，ProRoot MTA 的抗菌活性高于在血液中老化时。7 天后，抗菌活性显著降低。需要进一步评估以研究临床情况下的行为。
