Vo Quang Canh, Son Gitae, Seon Gyeung Mi, Um Sun Woo, Choi Sang Hoon, Yang Hyeong-Cheol
Department of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul, 03080, South Korea.
Sci Rep. 2025 Jan 2;15(1):478. doi: 10.1038/s41598-024-84183-9.
This study aimed to evaluate the effects of nanoparticulate CaCO (NPCC) on the biological properties of calcium silicate-based cements (CSCs), including their cytotoxicity, in vitro osteogenic activity, and interactions with rat femur tissue. The average size of NPCC was 90.3±26.0 nm. Cytotoxicity and osteogenic activity assays were performed using mouse bone marrow mesenchymal stem cells (BMSCs). BMSCs exposed to the eluents from CSC alone and CSC containing 2.5% NPCC (CSC-NPCC (2.5%)) for 24 h showed decreased cell viability at an eluent concentration of 75%. In contrast, CSC-NPCCs (5%, 10%, and 20%) did not affect cell viability. Regarding osteogenic activity, CSC-NPCCs (5%, 10%, 20%) enhanced the expression of osteogenic genes, including runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), type I collagen (COL-1), and osteocalcin (OCN). Additionally, mineralization in cell cultures was enhanced by CSC-NPCC, indicating that NPCC promoted the osteogenic activity of CSCs. In rat femurs, NPCC accelerates CSC resorption and stimulates bone regeneration at the implantation site. CSC alone occupied 22.2%±3.25% of the total femoral area at the implantation site, whereas CSC-NPCC (20%) occupied only 4%. These histological findings suggest that CSC-NPCC has potential as a biodegradable bone cement for use in bone defect areas that require regeneration.
本研究旨在评估纳米碳酸钙（NPCC）对硅酸钙基骨水泥（CSCs）生物学性能的影响，包括其细胞毒性、体外成骨活性以及与大鼠股骨组织的相互作用。NPCC的平均粒径为90.3±26.0纳米。使用小鼠骨髓间充质干细胞（BMSCs）进行细胞毒性和成骨活性测定。单独暴露于CSC洗脱液和含2.5%NPCC的CSC（CSC-NPCC（2.5%））洗脱液24小时的BMSCs，在洗脱液浓度为75%时细胞活力降低。相比之下，CSC-NPCCs（5%、10%和20%）对细胞活力没有影响。关于成骨活性，CSC-NPCCs（5%、10%、20%）增强了成骨基因的表达，包括 runt相关转录因子2（RUNX2）、碱性磷酸酶（ALP）、I型胶原蛋白（COL-1）和骨钙素（OCN）。此外，CSC-NPCC增强了细胞培养中的矿化作用，表明NPCC促进了CSCs的成骨活性。在大鼠股骨中，NPCC加速了CSC的吸收并刺激了植入部位的骨再生。单独的CSC在植入部位占股骨总面积的22.2%±3.25%，而CSC-NPCC（20%）仅占4%。这些组织学结果表明，CSC-NPCC有潜力作为一种可生物降解的骨水泥用于需要再生的骨缺损区域。
