Tokgöz Hüsnü, Taş Selim, Giray Özlem, Yalçınkaya Soner, Tokgöz Özlem, Koca Cemile, Savaş Murat, Erel Özcan
Department of Urology, Antalya Training and Research Hospital, Antalya, Turkey.
Department of Biochemistry , Antalya Training and Research Hospital, Antalya, Turkey.
Int Braz J Urol. 2017 May-Jun;43(3):455-461. doi: 10.1590/S1677-5538.IBJU.2016.0114.
The aim of this prospective clinical study was to investigate variations in a novel oxidative stress marker (thiol/disulphide homeostasis) in men who underwent transrectal ultrasound guided prostate biopsy (TRUSB).
A total of 22 men undergoing TRUSB of the prostate were enrolled in the study. Patients with abnormal digital rectal examination and/or total prostate specific antigen (PSA) over 4ng/mL underwent TRUSB with 12 cores. Serum samples were obtained before and just after the procedure to evaluate the possible changes in thiol/disulphide homeostasis. Mean age, total PSA and free PSA, prostate volume and histopathological data were also recorded.
Mean age of the study population was 65.05±8.89 years. Significant decreases in native and total thiol levels were documented after the biopsy procedure. However, serum disulphide levels and disulphide/native thiol, disulphide/total thiol and native / total thiol ratios did not significantly change after TRUSB. No correlation was observed between oxidative parameters and total PSA and free PSA levels, prostate volume and histopathology of the prostate. However, mean patient age was significantly correlated with mean native and total thiol levels.
Significant decreases in serum native and total thiol levels related to the prostate biopsy procedure suggest that TRUSB causes acute oxidative stress in the human body. Since our trial is the first in the current literature to investigate these oxidative stress markers in urology practice, additional studies are warranted.
这项前瞻性临床研究的目的是调查接受经直肠超声引导下前列腺穿刺活检（TRUSB）的男性体内一种新型氧化应激标志物（硫醇/二硫化物稳态）的变化情况。
共有22名接受前列腺TRUSB的男性纳入本研究。直肠指检异常和/或总前列腺特异性抗原（PSA）超过4ng/mL的患者接受12针TRUSB。在手术前和刚结束后采集血清样本，以评估硫醇/二硫化物稳态的可能变化。还记录了平均年龄、总PSA和游离PSA、前列腺体积及组织病理学数据。
研究人群的平均年龄为65.05±8.89岁。活检术后记录到天然硫醇和总硫醇水平显著下降。然而，TRUSB后血清二硫化物水平以及二硫化物/天然硫醇、二硫化物/总硫醇和天然/总硫醇比值未发生显著变化。氧化参数与总PSA和游离PSA水平、前列腺体积及前列腺组织病理学之间未观察到相关性。但是，患者平均年龄与平均天然硫醇和总硫醇水平显著相关。
与前列腺活检手术相关的血清天然硫醇和总硫醇水平显著下降表明，TRUSB会在人体内引起急性氧化应激。由于我们的试验是当前文献中首个在泌尿外科实践中研究这些氧化应激标志物的试验，因此有必要进行更多研究。
