Hanikoglu Ferhat, Hanikoglu Aysegul, Kucuksayan Ertan, Alisik Murat, Gocener Ahmet Anil, Erel Ozcan, Baykara Mehmet, Cuoghi Aurora, Tomasi Aldo, Ozben Tomris
a Biochemistry Laboratory , Kemer State Hospital , Antalya , Turkey.
b Department of Biochemistry, Medical Faculty , Akdeniz University , Antalya , Turkey.
Free Radic Res. 2016 Nov;50(sup1):S79-S84. doi: 10.1080/10715762.2016.1235787. Epub 2016 Oct 25.
Thiol groups are important anti-oxidants and essential molecules protecting organism against the harmful effects of reactive oxygen species (ROS). The aim of our study is to evaluate thiol-disulphide homeostasis with a novel recent automated method in patients with localized prostate cancer (PC) before and six months after radical prostatectomy (RP). 18 patients with PC and 17 healthy control subjects were enrolled into the study. Blood samples were collected from the controls subjects and patients before and six months after RP. Thiol-disulphide homeostasis was determined using a recently developed novel method. Prostate-specific antigen (PSA), albumin, total protein, total thiol, native thiol, disulphide and total antioxidant status (TAS) were measured and compared between the groups. Native thiol, total thiol and TAS levels were significantly higher in the control group than the patients before RP (p < .001). There was a non-significant increase in the native thiol, total thiol and TAS levels in the patients six months after RP in comparison to the levels before RP (p values .3, .3 and .09, respectively). We found a significant negative correlation between PSA and thiol levels. Our study demonstrated that the decreased thiol and TAS levels weakened anti-oxidant defence mechanism in the patients with PC as indicated. Increased oxidative stress in prostate cancer patients may cause metabolic disturbance and have a role in the aetiopathogenesis of prostate cancer.
巯基是重要的抗氧化剂，也是保护机体免受活性氧（ROS）有害影响的必需分子。我们研究的目的是采用一种最新的自动化方法，评估局限性前列腺癌（PC）患者在根治性前列腺切除术（RP）前及术后6个月的巯基-二硫键稳态。18例PC患者和17名健康对照者纳入本研究。在RP前及术后6个月从对照者和患者采集血样。采用最近开发的一种新方法测定巯基-二硫键稳态。测量并比较两组之间的前列腺特异性抗原（PSA）、白蛋白、总蛋白、总巯基、天然巯基、二硫键和总抗氧化状态（TAS）。对照组的天然巯基、总巯基和TAS水平显著高于RP前的患者（p＜0.001）。与RP前水平相比，患者在RP后6个月时天然巯基、总巯基和TAS水平有不显著的升高（p值分别为0.3、0.3和0.09）。我们发现PSA与巯基水平之间存在显著负相关。我们的研究表明，如所示，PC患者中巯基和TAS水平降低削弱了抗氧化防御机制。前列腺癌患者氧化应激增加可能导致代谢紊乱，并在前列腺癌的病因发病机制中起作用。
