Marcinowska A, Constantino-Casas F, Williams T, Hoather T, Blacklaws B, Dobson J
1 The Queen's Veterinary School Hospital, University of Cambridge, Cambridge, UK.
Vet Pathol. 2017 Jul;54(4):605-610. doi: 10.1177/0300985817690208. Epub 2017 Jan 27.
Flat-Coated Retriever dogs are predisposed to the development of histiocytic sarcoma (HS), a poorly differentiated, highly malignant neoplasm. The authors have previously documented a significant lymphocytic infiltrate in such tumors. The objective of this study was to examine the presence and expression of regulatory T cells in HS tumor samples. Forty tumors were included in this study. All tumors were immunolabeled for CD3, CD79a, CD25, CD45RA, and FOXP3. The proportion of positive cells was compared between tumors presenting as a localized primary soft tissue mass (soft tissue origin HS) and disseminated HS affecting viscera, especially the spleen (splenic origin HS). By immunohistochemistry, 95% of infiltrating T cells were positive for Foxp3 in all sections, suggesting the presence of regulatory T cells. The proportion of cells positive for FOXP3 was higher in the tumors arising in soft tissues, whereas the proportion of CD45RA-positive cells was higher in the splenic origin HS. Canine HS has an aggressive clinical behavior and is uniformly fatal. The difference in the proportion of tumor-infiltrating lymphocytes positive for these 2 markers in the 2 locations may represent differences in tumor microenvironment between the 2 sites.
平毛寻回犬易患组织细胞肉瘤（HS），这是一种低分化、高恶性肿瘤。作者之前已记录到此类肿瘤中有显著的淋巴细胞浸润。本研究的目的是检测HS肿瘤样本中调节性T细胞的存在及表达情况。本研究纳入了40个肿瘤样本。所有肿瘤样本均进行了CD3、CD79a、CD25、CD45RA和FOXP3的免疫标记。对表现为局限性原发性软组织肿块的肿瘤（软组织起源的HS）和累及内脏尤其是脾脏的播散性HS（脾起源的HS）之间的阳性细胞比例进行了比较。通过免疫组织化学检测，在所有切片中95%的浸润性T细胞Foxp3呈阳性，提示存在调节性T细胞。FOXP3阳性细胞比例在软组织起源的肿瘤中较高，而CD45RA阳性细胞比例在脾起源的HS中较高。犬HS具有侵袭性临床行为且无一例外均会致命。这两个部位这两种标志物阳性的肿瘤浸润淋巴细胞比例的差异可能代表了这两个部位肿瘤微环境的差异。
