Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Vet Pathol. 2011 May;48(3):764-71. doi: 10.1177/0300985810385153. Epub 2010 Oct 7.
The Flat Coated Retriever is a breed at risk of development of histiocytic sarcoma (HS), but in contrast to the disseminated form of disease recognized in the Bernese Mountain Dog, most reports of HS in Flat Coated Retrievers describe a localized lesion affecting the musculature or fascia of limbs. The purpose of this study was to review data and material received though an ongoing Flat Coated Retriever tumor survey to better define the presentation of HS in the breed and to determine the utility of subclassification of tumors arising at different sites by histology and immunohistologic phenotyping. Data on 180 dogs bearing HS-like tumors were available for review, which showed that although the majority (101 lesions, 57%) were primary limb lesions, 47 dogs (26%) had visceral, mainly splenic lesions with no peripheral primary tumor. A detailed histologic and immunohistologic review of 20 limb tumors and 20 splenic tumors showed that 2 distinct phenotypic subtypes could be identified: a histiocytic subtype, most prevalent in the splenic tumors, and a histiocytic-spindle-pleomorphic subtype, mainly seen in the limb tumors. Despite their variable morphology, all tumors expressed major histocompatibility complex class II and the leukocyte antigen CD18, but only those tumors in the spleen consistently expressed CD11d. The majority of tumors also contained a mild to moderate infiltrate of T lymphocytes.
平坦型可卡犬是一种容易发生组织细胞肉瘤（HS）的品种，但与伯尔尼山犬中发现的弥散性疾病形式不同，大多数平坦型可卡犬 HS 的报告描述了一种影响四肢肌肉或筋膜的局部病变。本研究的目的是通过正在进行的平坦型可卡犬肿瘤调查来回顾数据和材料，以更好地定义该品种 HS 的表现，并确定通过组织学和免疫组织化学表型对不同部位发生的肿瘤进行分类的实用性。共有 180 只患有 HS 样肿瘤的犬可供审查，结果表明，尽管大多数（101 个病变，57%）是原发性肢体病变，但 47 只犬（26%）有内脏病变，主要是脾脏病变，无外周原发性肿瘤。对 20 个肢体肿瘤和 20 个脾脏肿瘤进行详细的组织学和免疫组织化学研究表明，可以识别出 2 种不同的表型亚型：组织细胞亚型，在脾脏肿瘤中最为常见，以及组织细胞-梭形-多形性亚型，主要见于肢体肿瘤。尽管它们的形态学不同，但所有肿瘤都表达主要组织相容性复合体 II 类和白细胞抗原 CD18，但只有脾脏中的肿瘤始终表达 CD11d。大多数肿瘤还含有轻度至中度的 T 淋巴细胞浸润。
