College of Animal Science, Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, Guangdong 510642, China.
College of Forestry and Landscape Architecture, Guangdong Engineering &Research Center for Woody Fodder Plants, South China Agricultural University, Guangzhou, Guangdong 510642, China.
Sci Rep. 2017 Jan 30;7:41403. doi: 10.1038/srep41403.
Moringa oleifera (M. oleifera) is a remarkable species with high nutritional value and good biomass production, which can be used as livestock fodder. In this study, we examined changes in the faecal microbiota of thirty dairy cows in response to alternative M. oleifera diets and their effects on nutrient digestion, milk traits and the faecal concentrations of short-chain fatty acids. No differences in milk yield and constituents were found between the control and the M. oleifera alternative groups. Cows fed M. oleifera silage had lower dry matter digestibility, as well as the propionate and isovalerate concentrations in M. oleifera treated group. Using 16S rDNA gene sequencing, 1,299,556 paired-end reads were obtained. Clustering analysis revealed 13 phyla and 93 genera across all samples. Firmicutes and Bacteroidetes were the co-dominant phyla. Ten taxa displayed a significant difference in response to the high M. oleifera diet. In addition, strong correlations between Akkermansia and Prevotella with milk yield and protein indicated that some bacterial groups could be used to improve milk traits. Our results provided an insight into the microbiome-associated responses to M. oleifera in livestock diets, and could aid the development of novel applications of M. oleifera.
辣木（Moringa oleifera）是一种具有高营养价值和良好生物量生产能力的卓越物种，可用作牲畜饲料。在本研究中，我们研究了替代辣木叶饲料对 30 头奶牛粪便微生物群的影响及其对养分消化、牛奶特性和短链脂肪酸粪便浓度的影响。对照组和辣木叶替代组的牛奶产量和成分没有差异。饲喂辣木叶青贮料的奶牛的干物质消化率较低，且处理组中丙酸和异戊酸的浓度也较低。使用 16S rDNA 基因测序，获得了 1,299,556 对末端读取。聚类分析显示，所有样本中存在 13 个门和 93 个属。厚壁菌门和拟杆菌门是共同优势门。10 个分类群对高辣木叶饮食有显著差异的响应。此外，阿克曼氏菌和普雷沃氏菌与产奶量和蛋白质之间存在强烈相关性，表明一些细菌群可用于改善牛奶特性。我们的研究结果深入了解了辣木叶在牲畜饲料中的微生物组相关响应，并为辣木叶的新应用提供了参考。
