Rioux Renee A, Van Ryzin Benjamin J, Kerns James P
First author: Department of Plant Pathology, University of Wisconsin-Madison, Madison 53706; and second and third authors: Department of Entomology and Plant Pathology, North Carolina State University, Raleigh 27695.
Phytopathology. 2017 Jun;107(6):749-757. doi: 10.1094/PHYTO-08-16-0318-R. Epub 2017 Apr 12.
Brachypodium distachyon is a C3 grass that is an attractive model host system for studying pathogenicity of major turfgrass pathogens due to its genetic similarity to many cool-season turfgrasses. Infection assays with two or more isolates of the casual agents of dollar spot, brown patch, and Microdochium patch resulted in compatible interactions with B. distachyon inbred line Bd21-3. The symptoms produced by these pathogens on Bd21-3 closely resembled those observed on the natural turfgrass host (creeping bentgrass), demonstrating that B. distachyon is susceptible to the fungal pathogens that cause dollar spot, brown patch, and Microdochium patch on turfgrasses. The interaction between Sclerotinia homoeocarpa isolates and Brachypodium ecotypes was also investigated. Interestingly, differential responses of these ecotypes to S. homoeocarpa isolates was found, particularly when comparing B. distachyon to B. hybridum ecotypes. Taken together, these findings demonstrate that B. distachyon can be used as a model host system for these turfgrass diseases and leveraged for studies of molecular mechanisms contributing to host resistance.
二穗短柄草是一种C3禾本科植物，由于其与许多冷季型草坪草在遗传上相似，因此是研究主要草坪草病原体致病性的一个有吸引力的模式宿主系统。用两种或更多种币斑病、褐斑病和腐霉枯萎病病原菌分离物进行的侵染试验表明，这些病原菌与二穗短柄草自交系Bd21-3发生了亲和性互作。这些病原菌在Bd21-3上产生的症状与在天然草坪草宿主（匍匐翦股颖）上观察到的症状非常相似，这表明二穗短柄草对导致草坪草币斑病、褐斑病和腐霉枯萎病的真菌病原体敏感。还研究了核盘菌分离物与二穗短柄草生态型之间的相互作用。有趣的是，发现这些生态型对核盘菌分离物有不同的反应，特别是将二穗短柄草与杂交短柄草生态型进行比较时。综上所述，这些发现表明二穗短柄草可作为这些草坪草病害的模式宿主系统，并用于研究宿主抗性的分子机制。
