Department of Plant Pathology, The Ohio State University, Columbus, Ohio, United States of America.
PLoS One. 2012;7(8):e41150. doi: 10.1371/journal.pone.0041150. Epub 2012 Aug 8.
Sclerotinia homoeocarpa causes dollar spot disease, the predominate disease on highly-maintained turfgrass. Currently, there are major gaps in our understanding of the molecular interactions between S. homoeocarpa and creeping bentgrass. In this study, 454 sequencing technology was used in the de novo assembly of S. homoeocarpa and creeping bentgrass transcriptomes. Transcript sequence data obtained using Illumina's first generation sequencing-by-synthesis (SBS) were mapped to the transcriptome assemblies to estimate transcript representation in different SBS libraries. SBS libraries included a S. homoeocarpa culture control, a creeping bentgrass uninoculated control, and a library for creeping bentgrass inoculated with S. homoeocarpa and incubated for 96 h. A Fisher's exact test was performed to determine transcripts that were significantly different during creeping bentgrass infection with S. homoeocarpa. Fungal transcripts of interest included glycosyl hydrolases, proteases, and ABC transporters. Of particular interest were the large number of glycosyl hydrolase transcripts that target a wide range of plant cell wall compounds, corroborating the suggested wide host range and saprophytic abilities of S. homoeocarpa. Several of the multidrug resistance ABC transporters may be important for resistance to both fungicides and plant defense compounds. Creeping bentgrass transcripts of interest included germins, ubiquitin transcripts involved in proteasome degradation, and cinnamoyl reductase, which is involved in lignin production. This analysis provides an extensive overview of the S. homoeocarpa-turfgrass pathosystem and provides a starting point for the characterization of potential virulence factors and host defense responses. In particular, determination of important host defense responses may assist in the development of highly resistant creeping bentgrass varieties.
霍夫曼核盘菌引起钱斑病，是高度养护草坪的主要病害。目前，我们对霍夫曼核盘菌和匍匐翦股颖之间的分子相互作用的了解还存在很大的差距。在这项研究中，使用 454 测序技术对霍夫曼核盘菌和匍匐翦股颖的转录组进行从头组装。使用 Illumina 的第一代测序-合成（SBS）获得的转录序列数据被映射到转录组组装上，以估计不同 SBS 文库中的转录本表达。SBS 文库包括霍夫曼核盘菌培养对照、未接种匍匐翦股颖对照以及接种霍夫曼核盘菌并孵育 96 小时的匍匐翦股颖文库。使用 Fisher 精确检验确定在霍夫曼核盘菌感染匍匐翦股颖过程中差异显著的转录本。感兴趣的真菌转录本包括糖苷水解酶、蛋白酶和 ABC 转运蛋白。特别值得注意的是，大量靶向广泛植物细胞壁化合物的糖苷水解酶转录本，证实了霍夫曼核盘菌广泛的宿主范围和腐生能力。几种多药耐药 ABC 转运蛋白可能对杀菌剂和植物防御化合物的抗性都很重要。感兴趣的匍匐翦股颖转录本包括 germins、参与蛋白酶体降解的泛素转录本和参与木质素合成的肉桂酰还原酶。该分析提供了霍夫曼核盘菌-草坪病理系统的广泛概述，并为特征潜在的毒性因子和宿主防御反应提供了起点。特别是，确定重要的宿主防御反应可能有助于开发高度抗匍匐翦股颖品种。
