González-Peña Diana, Dudzik Danuta, García Antonia, Ancos Begoña de, Barbas Coral, Sánchez-Moreno Concepción
Institute of Food Science, Technology and Nutrition (ICTAN), Spanish National Research Council (CSIC), ES-28040 Madrid, Spain.
Center for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, San Pablo CEU University, Boadilla del Monte, ES-28668, Madrid, Spain.
Int J Mol Sci. 2017 Jan 28;18(2):267. doi: 10.3390/ijms18020267.
The consumption of functional ingredients has been suggested to be a complementary tool for the prevention and management of liver disease. In this light, processed onion can be considered as a source of multiple bioactive compounds with hepatoprotective properties. The liver fingerprint of male Wistar rats ( = 24) fed with three experimental diets (control (C), high-cholesterol (HC), and high-cholesterol enriched with onion (HCO) diets) was obtained through a non-targeted, multiplatform metabolomics approach to produce broad metabolite coverage. LC-MS, CE-MS and GC-MS results were subjected to univariate and multivariate analyses, providing a list of significant metabolites. All data were merged in order to figure out the most relevant metabolites that were modified by the onion ingredient. Several relevant metabolic changes and related metabolic pathways were found to be impacted by both HC and HCO diet. The model highlighted several metabolites (such as hydroxybutyryl carnitine and palmitoyl carnitine) modified by the HCO diet. These findings could suggest potential impairments in the energy-lipid metabolism, perturbations in the tricarboxylic acid cycle (TCA) cycle and β-oxidation modulated by the onion supplementation in the core of hepatic dysfunction. Metabolomics shows to be a valuable tool to evaluate the effects of complementary dietetic approaches directed to hepatic damage amelioration or non-alcoholic fatty liver disease (NAFLD) prevention.
食用功能性成分被认为是预防和管理肝脏疾病的一种补充手段。鉴于此，加工洋葱可被视为多种具有肝脏保护特性的生物活性化合物的来源。通过非靶向、多平台代谢组学方法，获得了喂食三种实验性饮食（对照（C）、高胆固醇（HC）和富含洋葱的高胆固醇（HCO）饮食）的雄性Wistar大鼠（n = 24）的肝脏指纹图谱，以实现广泛的代谢物覆盖。对液相色谱-质谱（LC-MS）、毛细管电泳-质谱（CE-MS）和气相色谱-质谱（GC-MS）结果进行单变量和多变量分析，得出一份重要代谢物清单。合并所有数据，以找出受洋葱成分影响的最相关代谢物。发现HC和HCO饮食均影响了一些相关的代谢变化和相关代谢途径。该模型突出了几种受HCO饮食影响的代谢物（如羟丁酰肉碱和棕榈酰肉碱）。这些发现可能表明，在肝功能障碍的核心部位，洋葱补充剂调节了能量-脂质代谢的潜在损伤、三羧酸循环（TCA）和β-氧化的紊乱。代谢组学被证明是评估针对改善肝损伤或预防非酒精性脂肪性肝病（NAFLD）的补充饮食方法效果的有价值工具。
