González-Peña Diana, Dudzik Danuta, Colina-Coca Clara, de Ancos Begoña, García Antonia, Barbas Coral, Sánchez-Moreno Concepción
Institute of Food Science, Technology and Nutrition (ICTAN), Spanish National Research Council (CSIC), José Antonio Novais 10, 28040, Madrid, Spain.
Center for Metabolomics and Bioanalysis (CEMBIO), Faculty of Pharmacy, San Pablo CEU University, Boadilla del Monte, 28668, Madrid, Spain.
Eur J Nutr. 2016 Apr;55(3):997-1010. doi: 10.1007/s00394-015-0914-1. Epub 2015 May 13.
The aim was to investigate the impact of hypercholesterolemic diet on the metabolome of male Wistar rats by a multiplatform metabolomic fingerprinting.
Male Wistar rats were fed with two different diets [control (C) and high-cholesterol diet (HC)-containing 2 % cholesterol and 0.5 % cholic acid]. After 7 weeks of experimental feeding, the rats were euthanized for blood collection and plasma recovery. The metabolite fingerprint was then achieved by applying a multiplatform comprising LC-MS, GC-MS and CE-MS.
Multivariate statistical analysis showed a clear separation between the C and HC groups. Individual differences in metabolites were evaluated using univariate statistical analysis, and multiple metabolites were identified and confirmed in the plasma. A global profiling integrates for the first time pathways affected by high-cholesterol diet intake and allowed us to elucidate some of the associated alterations underlying the hypercholesterolemia event in Wistar rats.
HC feeding stimulated the alteration of multiple pathways in Wistar rats, warning of the risk of developing important diseases, which can be modulated by the diet. Further studies are required to investigate the possibilities to revert or ameliorate the negative effects triggered by HC intake.
旨在通过多平台代谢组学指纹图谱研究高胆固醇饮食对雄性Wistar大鼠代谢组的影响。
给雄性Wistar大鼠喂食两种不同的饮食[对照（C）和含2%胆固醇及0.5%胆酸的高胆固醇饮食（HC）]。实验喂养7周后，对大鼠实施安乐死以采集血液并回收血浆。然后通过应用包括液相色谱 - 质谱联用（LC-MS）、气相色谱 - 质谱联用（GC-MS）和毛细管电泳 - 质谱联用（CE-MS）的多平台来获得代谢物指纹图谱。
多变量统计分析显示C组和HC组之间有明显区分。使用单变量统计分析评估代谢物的个体差异，并在血浆中鉴定和确认了多种代谢物。首次通过全面分析整合了受高胆固醇饮食摄入影响的途径，并使我们能够阐明Wistar大鼠高胆固醇血症事件背后的一些相关变化。
高胆固醇饮食喂养刺激了Wistar大鼠多种途径的改变，警示了发生重要疾病的风险，而这种风险可通过饮食进行调节。需要进一步研究以探讨逆转或改善高胆固醇饮食摄入引发的负面影响的可能性。
